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General Information



Gene_Symbol Gene_ID Gene_Name Chromosome Alias
WIF1 11197 WNT inhibitory factor 1  12WIF-1 







  • Evidence
  • Gene
  • mRNA
  • CDS
  • Protein
  • PTM
  • Enzyme
  • PPi
  • Pathway
  • Structural
  • Domain
  • Ontology
  • SNP
  • Drug
  • External Links



Evidence Information


Pubmed ID Year Sample Information Techniques Mechanism Fold Change Remark
15735684   2005Cell line : -

Source : -

Sample Type : -

No. of Sample : -

Stage : -

-Methylation--

16007117   2005Cell line : DLD 1, SW 48, and SW 480

Source : -

Sample Type : -

No. of Sample : -

Stage : -

RT-PCR, immunoblotting, and Methylation specific PCRMethylation-WIF-1 silencing due to promoter hypermethylation is an important mechanism underlying aberrant activation of the Wnt signaling pathway.

Modulation of the Wnt pathway, through reversal of WIF-1 silencing by demethylating agents, is a potential target for treatment and/or prevention of gastrointestinal cancers.

16491118   2006Cell line : DLD 1, SW 48, COLO 205, CT 26, SW 480, LS 174T, HT 29, LoVo, SW 620

Source : -

Sample Type : -

No. of Sample : -

Stage : -

Bisulfite genomic sequencing, Chromatin immunoprecipitation, Western blotting and immunofluorescenceMethylation-SFRP-1 and WIF-1 methylation-associated silencing occurred in all stages of colorectal tumorigenesis.

17923031   2007Cell line : HCT 116, SW 480

Source : -

Sample Type : CRC tumor and normal tissue

No. of Sample : -

Stage : -

Methylation-specific PCR, RT PCRMethylation-Hypermethylation of Wnt-antagonist genes is a common early event in the evolution of colorectal tumor. Methylation of sFRP1, 2, 5 and WIF-1 genes might serve as biomarkers for the early detection of colorectal tumor.

Methylation was more frequently found in colorectal tumors than in normal mucosa and adjacent normal mucosa from patients with tumor.

18542073   2008Cell line : -

Source : Wansbeck General Hospital (Ashington, Northumberland, UK).

Sample Type : -

No. of Sample : -

Stage : -

Bisulphite conversion of genomic DNA and analysis of CGI methylation, methylation specific PCR Methylation-CGI methylation of SFRP4, SFRP5 and WIF1 correctly identified 61.5% of polyp patients and 78.9% of neoplasia-free subject.

Methylation of SFRP4, SFRP5 and WIF1 genes may play a role in the earliest stages of the development of colorectal neoplasia.

Samples of mucosa and tumour tissue were obtained from a total of 19 patients colorectal cancer (11 males and 8 females) with a median age of 74 years. 13 adenomatous polyp patients were recruited (10 males and 3 females) with a median age of 67 years. The healthy and control 10 males and 10 females, median age 55 years .

19773381   2009Cell line : -

Source : Department of Surgery in the Samsung Medical Center, Seoul, Korea.

Sample Type : CRC tumor and normal tissue

No. of Sample : 243 colorectal cancer tissues, methylation

Stage : -

Methylation-specific PCR.Methylation-Tumor-specific methylation of APC, MGMT, RASSF2A, and Wif-1 genes might be a valuable biomarker in plasma for the early detection of colorectal cancer.


Gene Details



Chromosome: 12; NC_000012.11 (65444404..65515346, complement)

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Gene Type protein coding
Gene Family -
Description The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers. [provided by RefSeq, Jun 2010]  
Chromosome 12
Location 12q14.3
Start 65050624  
End 65121566  
Strand -  
Gene Sequence

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Length 70943  
OMIM ID 605186   
HGNC ID HGNC:18081   
Unigene ID Hs.284122    
Ensembl ID ENSG00000156076   
PharmGKB ID PA38291   

mRNA Information


mRNA ID NM_007191.4
Exon join(1..523,1011..1150,43713..43821,52663..52803, 53777..53872,54831..54926,58991..59086,65441..65536, 66354..66449,70097..70943)  
mRNA Sequence

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Length 2240  



CDS Information


   
Locationjoin(376..523,1011..1150,43713..43821,52663..52803, 53777..53872,54831..54926,58991..59086,65441..65536, 66354..66449,70097..70218)
CDS Sequence

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CCDS ID CCDS8971.1



Protein Information


      
Protein IDNP_009122.2
Protein NameWNT inhibitory factor 1
Molecular Wt. (Da)38431
Protein Sequence

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Length 379  
Uniprot IDP41161
Ensembl Protein ID ENSP00000306894  



Post-translational Modification Information (Source : HPRD)


No matching records could be found.

Enzyme Information


No matching records could be found.

PPi Information


(Click to view network : Source STRING)      View   

No matching records from HPRD source could be found.

Pathway Information


      
Pathway Name Pathway ID
Wnt signaling pathway hsa04310


Structure Information


No matching records could be found.

Domain Information




ID Location Description
smart0046935-179  WIF
cl0262335-179  WIF superfamily
pfam0201938-173  WIF


Ontology Information


   
HPRD ID592
Molecular ClassLigand
Molecular FunctionRNA binding (GO:0003723)
Biological ProcessRegulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolism (GO:0019219)  



SNP Information


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Drug Information


No matching records could be found.

External Links


                                 
ArrayExpressGO
ICGCGO
Cancer3DGO
COSMICGO
NCGGO
TSGeneGO
Tumor Gene Family of DatabasesGO
CCDBGO
G2SBCGO
Osteosarcoma DatabaseGO
RCDBGO