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General Information



Gene_Symbol Gene_ID Gene_Name Chromosome Alias
GAPDH 2597 Glyceraldehyde-3-phosphate dehydrogenase  12G3PD; GAPD  







  • Evidence
  • Gene
  • mRNA
  • CDS
  • Protein
  • PTM
  • Enzyme
  • PPi
  • Pathway
  • Structural
  • Domain
  • Ontology
  • SNP
  • Drug
  • External Links



Evidence Information


Pubmed ID Year Sample Information Techniques Mechanism Fold Change Remark
22350014   2012Cell line : -

Source : Tissus from Sun Yat-sen University Cancer Center and HCT116 and HT29 cell line.

Sample Type : -

No. of Sample : 195 CRC, including 28 patients with liver metastasis

Stage : TNM Stage I-IV

Cell culture, Western blotting,Assay of GAPDH and HK enzyme activities, Cell proliferation assay and Flow cytometryUp-regulation-High expression of GAPDH might play an important role in colon cancer development and metastasis.

GAPDH may be a potential target for colon cancer therapy.

3-bromopyruvate propyl ester (3-BrOP) preferentially inhibited GAPDH and exhibited potent activity in inducing colon cancer cell death by causing severe depletion of ATP. .

22911551   2012Cell line : -

Source : -

Sample Type : -

No. of Sample : -

Stage : -

-Up-regulation-GAPDH was commonly up-regulated in a variety of types of cancer, which was revealed to be potentially required for the cancer cell growth and tumor formation.

22991145   2012Cell line : -

Source : -

Sample Type : -

No. of Sample : -

Stage : -

Fluorogenic derivatization-liquid chromatography-tandem mass spectrometry.Up-regulation--


Gene Details



Chromosome: 12; NC_000012.11 (6643585..6647537)

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Gene Type protein coding
Gene Family -
Description The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]  
Chromosome 12
Location 12p13
Start 6643585  
End 6647537  
Strand +  
Gene Sequence

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Length 3953  
OMIM ID 138400   
HGNC ID HGNC:4141   
Unigene ID Hs.544577   
Ensembl ID ENSG00000111640   
PharmGKB ID -   

mRNA Information


mRNA ID NA
Exon NA  
mRNA Sequence

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Length 1401  



CDS Information


   
LocationNA
CDS Sequence

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CCDS ID NA



Protein Information


      
Protein IDNP_001243728.1
Protein NameGlyceraldehyde-3-phosphate dehydrogenase
Molecular Wt. (Da)31417
Protein Sequence

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Length 293  
Uniprot IDP36894
Ensembl Protein ID ENSP00000361107  



Post-translational Modification Information (Source : HPRD)


Residue Type site
CS-Nitrosylation152
CDisulfide Bridge152;156
YPhosphorylation42;-
SPhosphorylation83;-
SPhosphorylation151
TPhosphorylation182
TPhosphorylation184
TPhosphorylation211
TPhosphorylation154
KAcetylation61
KAcetylation186
KAcetylation84
CS-Nitrosylation156
CS-Nitrosylation247
TPhosphorylation246
TPhosphorylation59
TPhosphorylation75
SPhosphorylation210
TPhosphorylation176
SPhosphorylation25

Enzyme Information


   
EC1.2.1.9
Nameglyceraldehyde-3-phosphate dehydrogenase (NADP+)
triosephosphate dehydrogenase
dehydrogenase, glyceraldehyde phosphate (nicotinamide adenine dinucleotide phosphate)
glyceraldehyde phosphate dehydrogenase (NADP+)
glyceraldehyde 3-phosphate dehydrogenase (NADP+)
NADP+-glyceraldehyde phosphate dehydrogenase
NADP+-glyceraldehyde-3-phosphate dehydrogenase
glyceraldehyde-3-phosphate:NADP+ reductase
nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase
glyceraldehyde-3-phosphate dehydrogenase (NADP+)
ClassOxidoreductases; Acting on the aldehyde or oxo group of donors; With NAD+ or NADP+ as acceptor
SysnameD-glyceraldehyde-3-phosphate:NADP+ oxidoreductase  
SubstrateD-glyceraldehyde 3-phosphate [CPD:C00118]; NADP+ [CPD:C00006]; H2O  
Product3-phospho-D-glycerate [CPD:C00197]; NADPH [CPD:C00005]; H+  
Reaction (IUBMB)



PPi Information


(Click to view network : Source STRING)      View   

                                                                                                                                                                                                                                                                                                                                          
Interactant Gene ID (Source: HPRD) Gene Symbol HPRD ID Ref Seq ID Technique References
60ACTB32NP_001092.1in vitro11846445   
351APP100NP_000475.1in vitro8473906   
814CAMK47169NP_001735.1in vitro15680915   
816CAMK2B12127NP_001211.3in vitro;in vivo15199064   
3910LAMA42532NP_001098676.1yeast 2-hybrid16169070   
5050PAFAH1B34354NP_002564.1yeast 2-hybrid16169070   
5052PRDX11458NP_002565.1yeast 2-hybrid16169070   
5272SERPINB93480NP_004146.1yeast 2-hybrid16169070   
5717PSMD115119NP_002806.2yeast 2-hybrid16169070   
5862RAB2A1539NP_002856.1in vitro;in vivo15485821   
6118RPA21566NP_002937.1yeast 2-hybrid16169070   
6277S100A6237NP_055439.1in vitro1999197   
6477SIAH13736NP_001006611.1in vitro;in vivo;yeast 2-hybrid15951807   
6513SLC2A1683NP_006507.2in vitro2394733   
7083TK11771NP_003249.3yeast 2-hybrid16169070   
8536CAMK15412NP_003647.1in vitro;in vivo15680915   
10542HBXIP9773NP_006393.2yeast 2-hybrid16169070   
11261-6101NP_009167.1in vitro15312048   
22843PPM1E17888NP_055721.3in vitro;in vivo15680915   
54622ARL1512471NP_061960.1yeast 2-hybrid16169070   
55643BTBD212252NP_060267.2yeast 2-hybrid16169070   
56144PCDHA47322NP_061730.1yeast 2-hybrid16169070   


Pathway Information


                                    
Pathway Name Pathway ID
Alzheimer's disease hsa05010
Biosynthesis of amino acids hsa01230
Carbon metabolism hsa01200
Glycolysis / Gluconeogenesis hsa00010
HIF-1 signaling pathway hsa04066
Metabolic pathways hsa01100


Structure Information


PDB ID1U8F
Resolution1.75
MethodX-RAY DIFFRACTION
Chains O,P,Q  
Molecule Glyceraldehyde-3-phosphate dehydrogenase, liver  
Model View



Domain Information




ID Location Description
pfam02800157-314  Gp_dh_C
cl19935157-314  Gp_dh_C superfamily
smart008464-152  Gp_dh_N
cl228844-152  Gp_dh_N superfamily
pfam000444-152  Gp_dh_N
COG00573-335  GapA
PLN022722-332  PLN02272
PTZ000234-335  PTZ00023
TIGR015345-326  GAPDH-I
PLN023583-332  PLN02358
PRK154254-332  gapA
PRK077293-334  PRK07729
PTZ004341-335  PTZ00434
PRK074034-334  PRK07403
PRK089553-328  PRK08955
PLN030963-334  PLN03096
PLN022373-334  PLN02237
TIGR015325-326  E4PD_g-proteo
PRK135354-332  PRK13535
PRK0828910-332  PRK08289
PTZ003534-331  PTZ00353
COG013690-222  Asd
TIGR01296127-182  asd_B
TIGR00978145-282  asd_EA


Ontology Information


   
HPRD ID3192
Molecular ClassEnzyme: Dehydrogenase
Molecular FunctionReceptor signaling protein serine/threonine kinase activity (GO:0004702)
Biological ProcessCell communication (GO:0007154); Signal transduction (GO:0007165)  



SNP Information


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Drug Information


No matching records could be found.

External Links


                                 
ArrayExpressGO
ICGCGO
Cancer3DGO
COSMICGO
NCGGO
TSGeneGO
Tumor Gene Family of DatabasesGO
CCDBGO
G2SBCGO
Osteosarcoma DatabaseGO
RCDBGO