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General Information



Gene_Symbol Gene_ID Gene_Name Chromosome Alias
MMP9 4318 Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)  20GELB; CLG4B; MMP-9; MANDP2 







  • Evidence
  • Gene
  • mRNA
  • CDS
  • Protein
  • PTM
  • Enzyme
  • PPi
  • Pathway
  • Structural
  • Domain
  • Ontology
  • SNP
  • Drug
  • External Links



Evidence Information


Pubmed ID Year Sample Information Techniques Mechanism Fold Change Remark
17608852   2007Cell line : -

Source : -

Sample Type : CRC tumor and normal tissue

No. of Sample : 15 CRC representing primary tumor, adjacent normal colon, and liver metastases

Stage : TNM Stage I-IV

PCR-RFLPPolymorhpism-The frequency of MMP9-1,562 C homozygotes was significantly greater in colorectal cancer patients than in healthy controls.

17076885   2006Cell line : -

Source : -

Sample Type : CRC tumor and normal tissue

No. of Sample : 360 patients

Stage : TNM Stage I-III

-Unknown--

10952024   2000Cell line : -

Source : -

Sample Type : -

No. of Sample : -

Stage : -

Automated microtiter plate assay,In situ Hybridization, direct enzyme assay, immunohistochemistryUp-regulation-Matrix metalloproteases 1,2,9 were expressed at higher levels in the primary tumor than in adjacent normal tissue.

20001295   2010Cell line : -

Source : -

Sample Type : -

No. of Sample : -

Stage : -

Immunohistochemistry, multivariate survival (Cox) analysisUp-regulation-The detection of MMP-9 expression may be valuable in finding patients who are at high risk of developing disease recurrence.

In multivariate survival (Cox) analysis, MMP9 was a significant independent predictor of disease free survival, but not of disease specific survival.

23024755   2012Cell line : -

Source : -

Sample Type : CRC tumor and normal tissue

No. of Sample : 185 CRC and 304 healthy controls

Stage : TNM Stage I-IV

 Western blotting, ELISA, Immunohistochemistry and LC-MS/MS.Up-regulation-May play an essential role in local proteolysis of the extracellular matrics.

Allium fistulosum L., commonly known as scallion, is popularly used as a spice or vegetable worldwide, and as a traditional medicine in Asian cultures for treating a variety of diseases.

Scallion extracts inhibited the key inflammatory markers COX-2 and iNOS, and suppressed the expression of various cellular markers involved in tumor apoptosis, proliferation (cyclin D1 and c-Myc), angiogenesis (VEGF and HIF-1a), and tumor invasion (MMP-9 and ICAM-1).

CT-26 murine colorectal carcinoma cell line and BALB/c mice.

17912241   2007Cell line : -

Source : -

Sample Type : -

No. of Sample : -

Stage : -

ELISAUp-regulation-Patients with neoplasia had significantly elevated sMMP-9 levels (P<0.001).


Gene Details



Chromosome: 20; NC_000020.10 (44637547..44645200)

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Gene Type protein coding
Gene Family -
Description Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]  
Chromosome 20
Location 20q11.2-q1
Start 46008908  
End 46016561  
Strand +  
Gene Sequence

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Length 7654  
OMIM ID 120361   
HGNC ID HGNC:7176   
Unigene ID Hs.297413    
Ensembl ID ENSG00000100985   
PharmGKB ID -   

mRNA Information


mRNA ID NM_004994.2
Exon join(1..157,959..1191,1576..1724,2015..2143,2236..2409, 2667..2840,3230..3406,3520..3675,4348..4627,4750..4889, 5217..5367,5464..5567,7343..7654)  
mRNA Sequence

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Length 2387  



CDS Information


   
Location join(20..157,959..1191,1576..1724,2015..2143,2236..2409, 2667..2840,3230..3406,3520..3675,4348..4627,4750..4889, 5217..5367,5464..5567,7343..7461)
CDS Sequence

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CCDS ID CCDS13390.1



Protein Information


      
Protein IDNP_004985.2
Protein NameMatrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Molecular Wt. (Da)76371
Protein Sequence

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Length 707  
Uniprot IDP47902
Ensembl Protein ID ENSP00000231656  



Post-translational Modification Information (Source : HPRD)


Residue Type site
AProteolytic Cleavage93
RProteolytic Cleavage106
RProteolytic Cleavage106

Enzyme Information


   
EC3.4.24.35
Namegelatinase B
92-kDa gelatinase
matrix metalloproteinase 9
type V collagenase
92-kDa type IV collagenase
macrophage gelatinase
95 kDa type IV collagenase/gelatinase
collagenase IV
collagenase type IV
gelatinase MMP 9
MMP 9
matrix metalloproteinase 9
type IV collagen metalloproteinase
ClassHydrolases; Acting on peptide bonds (peptidases); Metalloendopeptidases
Sysname  
Substrate  
Product  
Reaction (IUBMB)



PPi Information


(Click to view network : Source STRING)      View   

                                                                                                                                                                                                                                                               
Interactant Gene ID (Source: HPRD) Gene Symbol HPRD ID Ref Seq ID Technique References
374AREG93NP_001648.1in vivo10389762   
960CD44115NP_000601.3in vivo1   065   227   117   912   430   
1277COL1A1362NP_000079.2in vivo9878537   
1278COL1A2363NP_000080.2in vivo9878537   
1282COL4A1359NP_001836.2in vivo9878537   
1284COL4A2355NP_001837.2in vivo9878537   
1285COL4A3354NP_000082.2in vivo9878537   
1286COL4A4360NP_000083.3in vivo9878537   
1404HAPLN1275NP_001875.1in vitro7694569   
2048EPHB22997NP_004433.2in vivo18713744   
2919CXCL11119NP_001502.1in vitro11023497   
3576IL8909NP_000575.1in vitro11023497   
3814KISS14475NP_002247.3in vitro;in vivo12879005   
5340PLG1417NP_000292.1in vitro9360944   
5858PZP7178NP_002855.2in vitro9344465   
6374CXCL58976NP_002985.1in vitro;in vivo1   295   025   710   095   770   
7035TFPI1064NP_006278.1in vitro10859319   


Pathway Information


                                                      
Pathway Name Pathway ID
Bladder cancer hsa05219
Estrogen signaling pathway hsa04915
Hepatitis B hsa05161
Leukocyte transendothelial migration hsa04670
MicroRNAs in cancer hsa05206
Pathways in cancer hsa05200
Proteoglycans in cancer hsa05205
TNF signaling pathway hsa04668
Transcriptional misregulation in cancer hsa05202


Structure Information


PDB ID1GKC
Resolution2.3
MethodX-RAY DIFFRACTION
Chains A,B  
Molecule 92 KDA TYPE IV COLLAGENASE  
Model View



Domain Information




ID Location Description
cd00094514-704  HX
cl02471514-704  HX superfamily
cd04278115-444  ZnMc_MMP
cl00064115-444  ZnMc superfamily
cd00062282-329  FN2
cl09951282-329  FN2 superfamily
cd00062224-271  FN2
cl09951224-271  FN2 superfamily
cd00062341-388  FN2
cl09951341-388  FN2 superfamily
pfam00040347-388  fn2
smart00059281-329  FN2
smart00059223-271  FN2
smart00059340-388  FN2
pfam00040288-329  fn2
pfam00040230-271  fn2
pfam04886472-506  PT
cl04822472-506  PT superfamily
cd04279382-444  ZnMc_MMP_like_1
pfam04886469-499  PT
smart00120521-565  HX
pfam00045521-564  Hemopexin
cd04277333-444  ZnMc_serralysin_like
cl00064333-444  ZnMc superfamily
pfam00045613-655  Hemopexin
smart00120613-658  HX
cd00203392-443  ZnMc
cd04268398-443  ZnMc_MMP_like
cd04277139-207  ZnMc_serralysin_like
pfam12200476-515  DUF3597
cl13612476-515  DUF3597 superfamily
PHA03185470-513  PHA03185
cl19236470-513  PHA03185 superfamily
pfam00413115-444  Peptidase_M10
smart00235112-218  ZnMc
smart00235398-444  ZnMc
PRK00404469-511  tatB
PHA03247469-526  PHA03247
PHA03377472-514  PHA03377


Ontology Information


   
HPRD ID313
Molecular ClassMetallo protease
Molecular FunctionDNA binding (GO:0003677)
Biological ProcessRegulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolism (GO:0019219)  



SNP Information


View SNP Geneview Report   

Drug Information


Uniprot ID DrugBank ID Name Type
P14780DB00786MarimastatSmallMoleculeDrug
P14780DB01017MinocyclineSmallMoleculeDrug
P14780DB01197CaptoprilSmallMoleculeDrug
P14780DB01296GlucosamineSmallMoleculeDrug

External Links


                                 
ArrayExpressGO
ICGCGO
Cancer3DGO
COSMICGO
NCGGO
TSGeneGO
Tumor Gene Family of DatabasesGO
CCDBGO
G2SBCGO
Osteosarcoma DatabaseGO
RCDBGO