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General Information



Gene_Symbol Gene_ID Gene_Name Chromosome Alias
NOS2A 4843 Nitric oxide synthase 2A (inducible, hepatocytes)  17NOS; INOS; NOS2A; HEP-NOS 







  • Evidence
  • Gene
  • mRNA
  • CDS
  • Protein
  • PTM
  • Enzyme
  • PPi
  • Pathway
  • Structural
  • Domain
  • Ontology
  • SNP
  • Drug
  • External Links



Evidence Information


Pubmed ID Year Sample Information Techniques Mechanism Fold Change Remark
11989855   2002Cell line : -

Source : -

Sample Type : -

No. of Sample : -

Stage : -

 Pre-embedding peroxidase-anti-peroxidase and post-embedding immunoelectron microscopic triple gold labelling techniques.Up-regulation-These data suggest that metastatic tumour vessel endothelium is characterized by increased expression of NOS2 and ET-1 and by decreases in NOS1 and NOS3.

12598314   2003Cell line : -

Source : Department of General Surgery, University of Florence, Florence, Italy.

Sample Type : CRC tumor

No. of Sample : 46

Stage : TNM Stage I-IV

Western blotting, Northern blotting, ImmunohistochemistryUp-regulation- Analyses confirmed the up-regulation of the iNOS protein and gene in the tumor specimens as compared with normal mucosa.

15695237   2005Cell line : -

Source : Department of Internal Medicine, Sapporo Medical University, Chuo-ku, Sapporo, Japan and Department of Clinical Laboratory Science, Yamaguchi University School of Medicine, Japan.

Sample Type : CRC tumor

No. of Sample : 90 colorectal tumors, including 63 adenomas and 27 cancers

Stage : TNM Stage I-IV

real time RT-PCR, immunohistochemistry, Northern blottingUp-regulation- Results suggest that E1AF, in conjunction with the expression of MMP-1, MMP-7, COX-2 and iNOS, plays an important role in the early stage of colorectal carcinogenesis.

15922861   2005Cell line : -

Source : County Hospital Ryhov, Jo�nko�ping, Sweden.

Sample Type : CRC Blood

No. of Sample : 131

Stage : -

PCR , Genotyping,SequencingUp-regulation- Increased expression of nitric oxide synthase 2 (NOS2) in colorectal cancer (CRC) has been identified.NOS2 promoter polymorphisms have a limited effect on the onset or progression of CRC.


Gene Details



Chromosome: 17; NC_000017.10 (26083792..26127555, complement)

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Gene Type protein coding
Gene Family -
Description Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]  
Chromosome 17
Location 17q11.2-q1
Start 27756766  
End 27804341  
Strand -  
Gene Sequence

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Length 47576  
OMIM ID 163730   
HGNC ID HGNC:7873   
Unigene ID Hs.709191   
Ensembl ID ENSG00000007171   
PharmGKB ID PA25376   

mRNA Information


mRNA ID XM_011524859.1
Exon join(1..219,5460..5642,14654..14738,15411..15533, 16516..16664,21236..21398,22236..22327,23165..23306, 23436..23575,25286..25460,25551..25652,29891..30085, 31099..31181,31890..32034,33325..33429,34758..34807, 35191..35365,36505..36637,37754..37832,38626..38807, 40198..40361,41337..41544,43111..43198,43598..43719, 44164..44312,45267..45461,46989..47576)  
mRNA Sequence

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Length 4206  



CDS Information


   
Location join(5533..5642,14654..14738,15411..15533,16516..16664, 21236..21398,22236..22327,23165..23306,23436..23575, 25286..25460,25551..25652,29891..30085,31099..31181, 31890..32034,33325..33429,34758..34807,35191..35365, 36505..36637,37754..37832,38626..38735,40198..40361, 41337..41544,43111..43198,43598..43719,44164..44312, 45267..45461,46989..47096)
CDS Sequence

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CCDS ID -



Protein Information


      
Protein IDNP_000616.3
Protein NameNitric oxide synthase 2A (inducible, hepatocytes)
Molecular Wt. (Da)130987
Protein Sequence

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Length 1153  
Uniprot IDP10909
Ensembl Protein ID ENSP00000315130  



Post-translational Modification Information (Source : HPRD)


Residue Type site
YNitration299
YNitration336
YNitration446
YNitration698
YNitration297
YNitration407
YNitration659

Enzyme Information


No matching records could be found.

PPi Information


(Click to view network : Source STRING)      View   

                              
Interactant Gene ID (Source: HPRD) Gene Symbol HPRD ID Ref Seq ID Technique References
60ACTB32NP_001092.1in vitro12080081   
5880RAC23628NP_002863.1in vitro;in vivo;yeast 2-hybrid11457725   


Pathway Information


                                                                                          
Pathway Name Pathway ID
Amoebiasis hsa05146
Arginine and proline metabolism hsa00330
Arginine biosynthesis hsa00220
Calcium signaling pathway hsa04020
Chagas disease (American trypanosomiasis) hsa05142
HIF-1 signaling pathway hsa04066
Leishmaniasis hsa05140
Metabolic pathways hsa01100
Pathways in cancer hsa05200
Peroxisome hsa04146
Pertussis hsa05133
Salmonella infection hsa05132
Small cell lung cancer hsa05222
Toxoplasmosis hsa05145
Tuberculosis hsa05152


Structure Information


PDB ID1NSI
Resolution2.55
MethodX-RAY DIFFRACTION
Chains A,B,C  
Molecule PROTEIN (NITRIC OXIDE SYNTHASE)  
Model View



Domain Information




ID Location Description
cd0079584-496  NOS_oxygenase_euk
cl0025484-496  NOS_oxygenase superfamily
cd00575138-492  NOS_oxygenase
pfam02898131-496  NO_synthase
cd06202736-1132  Nitric_oxide_synthase
cl06868736-1132  FNR_like superfamily
cd00794139-492  NOS_oxygenase_prok
COG4362139-492  COG4362
cd06204729-1127  CYPOR
pfam00667727-947  FAD_binding_1
cl19189727-947  FAD_binding_1 superfamily
cd06199736-1128  SiR
cd06207736-1123  CyPoR_like
cd06182863-1128  CYPOR_like
cd06206743-1128  bifunctional_CYPOR
cd06203737-1127  methionine_synthase_red
pfam00258541-672  Flavodoxin_1
cl00438541-672  FMN_red superfamily
cd06208904-1124  CYPOR_like_FNR
cd06201871-1128  SiR_like2
cd00322878-1102  FNR_like
cd06200889-1128  SiR_like1
pfam00175979-1092  NAD_binding_1
cd06195906-1098  FNR1
cd06188906-1098  NADH_quinone_reductase
cd06214905-1098  PA_degradation_oxidoreductase_like
cd06217906-1101  FNR_iron_sulfur_binding_3
PRK08105582-660  PRK08105
cd06216906-1083  FNR_iron_sulfur_binding_2
PRK06703538-675  PRK06703
PRK09004577-655  PRK09004
cd06184905-1098  flavohem_like_fad_nad_binding
cd06187905-1098  O2ase_reductase_like
cd06198908-1111  FNR_like_3
cd06183942-1110  cyt_b5_reduct_like
cd06194905-1092  FNR_N-term_Iron_sulfur_binding
cd06221909-1036  sulfite_reductase_like
cd06218947-1097  DHOD_e_trans
cd06191906-1109  FNR_iron_sulfur_binding
cd06215906-1035  FNR_iron_sulfur_binding_1
cd06189904-1104  flavin_oxioreductase
COG0716538-679  FldA
cd06196938-1111  FNR_like_1
cd06209942-1098  BenDO_FAD_NAD
COG0369538-1127  CysJ
TIGR01931538-1128  cysJ
PRK10953534-1128  cysJ
PRK06214730-1128  PRK06214
TIGR03224906-1124  benzo_boxA
PLN03116904-1128  PLN03116
PLN03115904-1128  PLN03115
COG0543872-1138  Mcr1
COG1018906-1098  Fpr
COG2871906-1028  NqrF
COG4097978-1109  COG4097
TIGR01941906-1098  nqrF
PRK07609967-1034  PRK07609
PRK10926942-1097  PRK10926
PRK05464942-1034  PRK05464
PRK08345942-1095  PRK08345


Ontology Information


   
HPRD ID8450
Molecular ClassEnzyme: Synthase
Molecular FunctionChaperone activity (GO:0003754)
Biological ProcessProtein metabolism (GO:0019538)  



SNP Information


View SNP Geneview Report   

Drug Information


Uniprot ID DrugBank ID Name Type
P35228DB00997DoxorubicinSmallMoleculeDrug
P35228DB00125L-ArginineSmallMoleculeDrug
P35228DB00155L-CitrullineSmallMoleculeDrug
P35228DB01110MiconazoleSmallMoleculeDrug
P35228DB01234DexamethasoneSmallMoleculeDrug
P35228DB08814TriflusalSmallMoleculeDrug

External Links


                                 
ArrayExpressGO
ICGCGO
Cancer3DGO
COSMICGO
NCGGO
TSGeneGO
Tumor Gene Family of DatabasesGO
CCDBGO
G2SBCGO
Osteosarcoma DatabaseGO
RCDBGO