Search results:


General Information



Gene_Symbol Gene_ID Gene_Name Chromosome Alias
ARHGEF12 23365 Rho guanine nucleotide exchange factor (GEF) 12  11LARG; PRO2792 







  • Evidence
  • Gene
  • mRNA
  • CDS
  • Protein
  • PTM
  • Enzyme
  • PPi
  • Pathway
  • Structural
  • Domain
  • Ontology
  • SNP
  • Drug
  • External Links



Evidence Information


Pubmed ID Year Sample Information Techniques Mechanism Fold Change Remark
19734946   2009Cell line : -

Source : Singapore General Hospital.

Sample Type : CRC tumor and normal tissue

No. of Sample : 30

Stage : -

Microsatellite analysis, Assessment of LOH, Array CGH, Fluorescence in situ hybridization, Real-time RT-PCRDown-regulation-Enforced expression of LARG in breast and colorectal cancer cells by stable transfection resulted in reduced cell proliferation and colony formation, as well as in a markedly slower cell migration rate in colorectal cancer cells, providing functional evidence for LARG as a candidate tumor suppressor gene.

LARG frequently underexpressed in colorectal carcinomas with a reduced expression 5 of 10 (50%) colorectal cell lines and in 20 of 37 (54%) primary colorectal cancers.


Gene Details



Chromosome: 11; NC_000011.9 (120207618..120360645)

View Larger Image
   
Gene Type protein coding
Gene Family Rho guanine nucleotide exchange factors
Description Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]  
Chromosome 11
Location 11q23.3
Start 120336406  
End 120489936  
Strand -  
Gene Sequence

   Download   Select all

Length 153531  
OMIM ID 604763   
HGNC ID HGNC:14193   
Unigene ID Hs.24598    
Ensembl ID ENSG00000196914   
PharmGKB ID PA24969   

mRNA Information


mRNA ID XM_011542720.1
Exon join(1..271,69713..69736,71333..71418,72989..73045, 84348..84446,85398..85447,87953..88010,91664..91842, 93035..93112,93307..93426,95366..95506,100903..100977, 103724..103816,105302..105412,105699..105797, 109017..109059,109998..110103,110543..110680, 111469..111501,111829..111943,112704..112809, 115107..115319,120713..120845,121316..121351, 121675..121829,122769..122915,124267..124352, 128832..128957,130789..130903,132883..132983, 136645..136722,138155..138230,138935..139102, 140256..140343,140814..140900,141042..141121, 141751..141984,143555..144025,144855..145171, 148033..148102,148662..153527)  
mRNA Sequence

   Download   Select all

Length 9729  



CDS Information


   
Location join(839..870,69713..69736,71333..71418,72989..73045, 84348..84446,85398..85447,87953..88010,91664..91842, 93035..93112,93307..93426,95366..95506,100903..100977, 103724..103816,105302..105412,105699..105797, 109017..109059,109998..110103,110543..110680, 111469..111501,111829..111943,112704..112809, 115107..115319,120713..120845,121316..121351, 121675
CDS Sequence

   Download   Select all

CCDS ID CCDS41727.1



Protein Information


      
Protein IDNP_001185594.1
Protein NameRho guanine nucleotide exchange factor (GEF) 12
Molecular Wt. (Da)170985
Protein Sequence

   Download   Select all

Length 1525  
Uniprot IDQ16394
Ensembl Protein ID ENSP00000367446  



Post-translational Modification Information (Source : HPRD)


Residue Type site
SPhosphorylation341
SPhosphorylation1288;-
TPhosphorylation1286
SAcetylation2
YPhosphorylation546
TPhosphorylation736
SPhosphorylation22
SPhosphorylation190
SPhosphorylation637

Enzyme Information


No matching records could be found.

PPi Information


(Click to view network : Source STRING)      View   

                                                                                          
Interactant Gene ID (Source: HPRD) Gene Symbol HPRD ID Ref Seq ID Technique References
387RHOA1323NP_001655.1in vitro;in vivo11373293   
2768GNA125093NP_031379.2in vitro;in vivo1   251   586   611   094   160   
3480IGF1R932NP_000866.1in vitro;in vivo11724822   
5364PLXNB13032NP_002664.2in vitro;in vivo;yeast 2-hybrid12220504   
10672GNA135100NP_006563.2in vitro;in vivo1   251   586   611   094   160   
23654PLXNB25050NP_036533.2in vitro;yeast 2-hybrid12183458   


Pathway Information


                                          
Pathway Name Pathway ID
Axon guidance hsa04360
Pathways in cancer hsa05200
Platelet activation hsa04611
Proteoglycans in cancer hsa05205
Regulation of actin cytoskeleton hsa04810
Tuberculosis hsa05152
Vascular smooth muscle contraction hsa04270


Structure Information


PDB ID1TXD
Resolution2.13
MethodX-RAY DIFFRACTION
Chains A  
Molecule Rho guanine nucleotide exchange factor 12  
Model View



Domain Information




ID Location Description
cd08754349-570  RGS_LARG
cl02565349-570  RGS superfamily
cd13390995-1132  PH_LARG
cl17171995-1132  PH-like superfamily
cd00160788-975  RhoGEF
cl02571788-975  RhoGEF superfamily
cd0099271-145  PDZ_signaling
cl0011771-145  PDZ superfamily
pfam09128368-558  RGS-like
cd08736379-498  RGS_RhoGEF-like
cd08755378-560  RGS_p115RhoGEF
pfam00621791-976  RhoGEF
cd13391993-1127  PH_PRG
smart00325791-976  RhoGEF
cd146791011-1127  PH_p115RhoGEF
cd133291020-1131  PH_RhoGEF
cd08753350-491  RGS_PDZRhoGEF
smart0022869-149  PDZ
cd08756379-491  RGS_GEF_like
cd157941017-1146  PH_ARHGEF18
pfam0059573-145  PDZ
cd0013681-144  PDZ
cd146801020-1127  PH_p190RhoGEF
cd0098784-145  PDZ_serine_protease
cd0098881-153  PDZ_CTP_protease
cd0098981-153  PDZ_metalloprotease
cd133931017-1127  PH_ARHGEF2
pfam1318081-145  PDZ_2
cd07440379-487  RGS
cd157891020-1127  PH_ARHGEF2_18_like
cd133921020-1127  PH_AKAP13
COG5422737-1026  ROM1
COG079381-154  CtpA
TIGR0022581-154  prc
COG026565-164  DegQ
TIGR0203792-145  degP_htrA_DO
TIGR0203792-144  degP_htrA_DO
PRK1077991-124  PRK10779


Ontology Information


   
HPRD ID612
Molecular ClassGuanine nucleotide exchange factor
Molecular FunctionTransporter activity (GO:0005215)
Biological ProcessTransport (GO:0006810)  



SNP Information


View SNP Geneview Report   

Drug Information


No matching records could be found.

External Links


                                 
ArrayExpressGO
ICGCGO
Cancer3DGO
COSMICGO
NCGGO
TSGeneGO
Tumor Gene Family of DatabasesGO
CCDBGO
G2SBCGO
Osteosarcoma DatabaseGO
RCDBGO