Search results:
General Information
| Gene_Symbol | Gene_ID | Gene_Name | Chromosome | Alias |
|---|---|---|---|---|
| ARHGEF12 | 23365 | Rho guanine nucleotide exchange factor (GEF) 12  | 11 | LARG; PRO2792  |
- Evidence
- Gene
- mRNA
- CDS
- Protein
- PTM
- Enzyme
- PPi
- Pathway
- Structural
- Domain
- Ontology
- SNP
- Drug
- External Links
Evidence Information
| Pubmed ID | Year | Sample Information | Techniques | Mechanism | Fold Change | Remark |
|---|---|---|---|---|---|---|
| 19734946    | 2009 | Cell line : - Source : Singapore General Hospital. Sample Type : CRC tumor and normal tissue No. of Sample : 30 Stage : - | Microsatellite analysis, Assessment of LOH, Array CGH, Fluorescence in situ hybridization, Real-time RT-PCR | Down-regulation | - | Enforced expression of LARG in breast and colorectal cancer cells by stable transfection resulted in reduced cell proliferation and colony formation, as well as in a markedly slower cell migration rate in colorectal cancer cells, providing functional evidence for LARG as a candidate tumor suppressor gene. LARG frequently underexpressed in colorectal carcinomas with a reduced expression 5 of 10 (50%) colorectal cell lines and in 20 of 37 (54%) primary colorectal cancers. |
Gene Details

| Gene Type | protein coding |
|---|---|
| Gene Family | Rho guanine nucleotide exchange factors |
| Description | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]   | Chromosome | 11 |
| Location | 11q23.3 |
| Start | 120336406   |
| End | 120489936   |
| Strand | -   |
| Gene Sequence |    Download   Select all |
| Length | 153531   |
| OMIM ID | 604763    |
| HGNC ID | HGNC:14193    |
| Unigene ID | Hs.24598     |
| Ensembl ID | ENSG00000196914    |
| PharmGKB ID | PA24969    |
mRNA Information
| mRNA ID | XM_011542720.1 |
|---|---|
| Exon | join(1..271,69713..69736,71333..71418,72989..73045, 84348..84446,85398..85447,87953..88010,91664..91842, 93035..93112,93307..93426,95366..95506,100903..100977, 103724..103816,105302..105412,105699..105797, 109017..109059,109998..110103,110543..110680, 111469..111501,111829..111943,112704..112809, 115107..115319,120713..120845,121316..121351, 121675..121829,122769..122915,124267..124352, 128832..128957,130789..130903,132883..132983, 136645..136722,138155..138230,138935..139102, 140256..140343,140814..140900,141042..141121, 141751..141984,143555..144025,144855..145171, 148033..148102,148662..153527)   | mRNA Sequence |    Download   Select all |
| Length | 9729   |
CDS Information
| Location | join(839..870,69713..69736,71333..71418,72989..73045, 84348..84446,85398..85447,87953..88010,91664..91842, 93035..93112,93307..93426,95366..95506,100903..100977, 103724..103816,105302..105412,105699..105797, 109017..109059,109998..110103,110543..110680, 111469..111501,111829..111943,112704..112809, 115107..115319,120713..120845,121316..121351, 121675 |
|---|---|
| CDS Sequence |    Download   Select all |
| CCDS ID | CCDS41727.1 |    
Protein Information
| Protein ID | NP_001185594.1 |
|---|---|
| Protein Name | Rho guanine nucleotide exchange factor (GEF) 12 |
| Molecular Wt. (Da) | 170985 |
| Protein Sequence |    Download   Select all |
| Length | 1525   |
| Uniprot ID | Q16394 |    
| Ensembl Protein ID | ENSP00000367446   |    
Post-translational Modification Information (Source : HPRD)
| Residue | Type | site |
|---|---|---|
| S | Phosphorylation | 341 |
| S | Phosphorylation | 1288;- |
| T | Phosphorylation | 1286 |
| S | Acetylation | 2 |
| Y | Phosphorylation | 546 |
| T | Phosphorylation | 736 |
| S | Phosphorylation | 22 |
| S | Phosphorylation | 190 |
| S | Phosphorylation | 637 |
Enzyme Information
No matching records could be found.
PPi Information
(Click to view network : Source STRING)      View   
| Interactant Gene ID (Source: HPRD) | Gene Symbol | HPRD ID | Ref Seq ID | Technique | References |
|---|---|---|---|---|---|
| 387 |    RHOA |    1323 |    NP_001655.1 |    in vitro;in vivo |    11373293    |
| 2768 |    GNA12 |    5093 |    NP_031379.2 |    in vitro;in vivo |    1   251   586   611   094   160    |
| 3480 |    IGF1R |    932 |    NP_000866.1 |    in vitro;in vivo |    11724822    |
| 5364 |    PLXNB1 |    3032 |    NP_002664.2 |    in vitro;in vivo;yeast 2-hybrid |    12220504    |
| 10672 |    GNA13 |    5100 |    NP_006563.2 |    in vitro;in vivo |    1   251   586   611   094   160    |
| 23654 |    PLXNB2 |    5050 |    NP_036533.2 |    in vitro;yeast 2-hybrid |    12183458    |
Pathway Information
| Pathway Name | Pathway ID |
|---|---|
| Axon guidance |    hsa04360 |    
| Pathways in cancer |    hsa05200 |    
| Platelet activation |    hsa04611 |    
| Proteoglycans in cancer |    hsa05205 |    
| Regulation of actin cytoskeleton |    hsa04810 |    
| Tuberculosis |    hsa05152 |    
| Vascular smooth muscle contraction |    hsa04270 |    
Structure Information
| PDB ID | 1TXD |
|---|---|
| Resolution | 2.13 |
| Method | X-RAY DIFFRACTION |
| Chains | A   |
| Molecule | Rho guanine nucleotide exchange factor 12   |
| Model | View |
Domain Information
| ID | Location | Description |
|---|---|---|
| cd08754 | 349-570  | RGS_LARG |
| cl02565 | 349-570  | RGS superfamily |
| cd13390 | 995-1132  | PH_LARG |
| cl17171 | 995-1132  | PH-like superfamily |
| cd00160 | 788-975  | RhoGEF |
| cl02571 | 788-975  | RhoGEF superfamily |
| cd00992 | 71-145  | PDZ_signaling |
| cl00117 | 71-145  | PDZ superfamily |
| pfam09128 | 368-558  | RGS-like |
| cd08736 | 379-498  | RGS_RhoGEF-like |
| cd08755 | 378-560  | RGS_p115RhoGEF |
| pfam00621 | 791-976  | RhoGEF |
| cd13391 | 993-1127  | PH_PRG |
| smart00325 | 791-976  | RhoGEF |
| cd14679 | 1011-1127  | PH_p115RhoGEF |
| cd13329 | 1020-1131  | PH_RhoGEF |
| cd08753 | 350-491  | RGS_PDZRhoGEF |
| smart00228 | 69-149  | PDZ |
| cd08756 | 379-491  | RGS_GEF_like |
| cd15794 | 1017-1146  | PH_ARHGEF18 |
| pfam00595 | 73-145  | PDZ |
| cd00136 | 81-144  | PDZ |
| cd14680 | 1020-1127  | PH_p190RhoGEF |
| cd00987 | 84-145  | PDZ_serine_protease |
| cd00988 | 81-153  | PDZ_CTP_protease |
| cd00989 | 81-153  | PDZ_metalloprotease |
| cd13393 | 1017-1127  | PH_ARHGEF2 |
| pfam13180 | 81-145  | PDZ_2 |
| cd07440 | 379-487  | RGS |
| cd15789 | 1020-1127  | PH_ARHGEF2_18_like |
| cd13392 | 1020-1127  | PH_AKAP13 |
| COG5422 | 737-1026  | ROM1 |
| COG0793 | 81-154  | CtpA |
| TIGR00225 | 81-154  | prc |
| COG0265 | 65-164  | DegQ |
| TIGR02037 | 92-145  | degP_htrA_DO |
| TIGR02037 | 92-144  | degP_htrA_DO |
| PRK10779 | 91-124  | PRK10779 |
Ontology Information
| HPRD ID | 612 |    
|---|---|
| Molecular Class | Guanine nucleotide exchange factor |
| Molecular Function | Transporter activity (GO:0005215) |
| Biological Process | Transport (GO:0006810)   |
Drug Information
No matching records could be found.
External Links
| ArrayExpress | GO |    
|---|---|
| ICGC | GO |    
| Cancer3D | GO |    
| COSMIC | GO |    
| NCG | GO |    
| TSGene | GO |    
| Tumor Gene Family of Databases | GO |    
| CCDB | GO |    
| G2SBC | GO |    
| Osteosarcoma Database | GO |    
| RCDB | GO |    
