Search results:
General Information
| Gene_Symbol | Gene_ID | Gene_Name | Chromosome | Alias |
|---|---|---|---|---|
| SMAD3 | 4088 | SMAD family member 3  | 15 | LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436  |
- Evidence
- Gene
- mRNA
- CDS
- Protein
- PTM
- Enzyme
- PPi
- Pathway
- Structural
- Domain
- Ontology
- SNP
- Drug
- External Links
Evidence Information
| Pubmed ID | Year | Sample Information | Techniques | Mechanism | Fold Change | Remark |
|---|---|---|---|---|---|---|
| 22278155    | 2012 | Cell line : - Source : Tzaneio General Hospital, Pireaus, Greece. Sample Type : CRC tumor and normal tissue No. of Sample : 195 unrelated CRC specimens Stage : TNM Stage I-IV | Immunohistochemistry | Down-regulation | - | TGF-B, TGF-BR2, Smad4, pSmad2/3, and E-cadherin emerge as valuable independent biomarkers of prognosis in CRC patients. TGF-B, TGF-BR1, TGF-BR2, Smad4, pSmad2/3, and E-cadherin are closely related to TNM stage of CRC. |
| 16828225    | 2007 | Cell line : SNU 61, SNU 407, SNU 769A, SNU 769B, SNU 1047, SNU 81, SNU 175 and A202V, SNU 1040, and SNU 769A. Source : KCLB (the Korean Cell Line Bank) (Seoul, Korea). Sample Type : - No. of Sample : - Stage : - | - | Mutation | - | A missense mutation (R373H) in Smad3 gene was found in SNU-769A. This mutation was found to result in the inhibition of translocation of Smad3 protein to the nucleus and a reduction in the activity of Smad3 during TGF-beta-induced transcriptional activation.
|
| 23139211    | 2012 | Cell line : 36 CRC cell lines Source : - Sample Type : CRC tumor and normal tissue No. of Sample : 744 primary CRCs Stage : - | SNP microarray | Mutation | - | 744 primary CRCs and 36 CRC cell lines were sequenced for SMAD4, SMAD2, and SMAD3 and analyzed for allelic loss by single-nucleotide polymorphism (SNP) microarray analysis. In silico analyses suggested the majority of the mutations were pathogenic, with most missense changes predicted to reduce protein stability or hinder SMAD complex formation. |
| 15665291    | 2005 | Cell line : - Source : Department of Surgical Pathology at Kansai Medical University Hospital. Sample Type : CRC tumor and normal tissue No. of Sample : - Stage : - | RT-PCR, Immunohistochemistry, Immunoprecipitation and Immunoblotting, In vitro Kinase Assay | Phosphorylation | - | Acceleration of Smad2 and Smad3 phosphorylation via c-Jun NH(2)-terminal kinase during human colorectal carcinogenesis.
|
| 16528675    | 2006 | Cell line : - Source : - Sample Type : - No. of Sample : - Stage : - | - | Phosphorylation | - | TGF-beta signaling involves phosphorylation of Smad3 at serine residues 208 and 213 in the linker region and serine residues 423 and 425 in the C-terminal region.
|
| 17093904    | 2006 | Cell line : - Source : - Sample Type : - No. of Sample : - Stage : - | - | Phosphorylation | - | During sporadic human colorectal carcinogenesis, TGF-beta signaling confers a selective advantage upon tumor cells by shifting from epithelial Tbeta RI/pSmad3C pathway to mesenchymal JNK/pSmad3L pathway. Specific inhibition of the JNK/pSmad3L pathway will become a therapy for human colorectal cancer that restores the lost tumor-suppressive function observed in normal colorectal epithelial cells. |
| 19531654    | 2009 | Cell line : ACBRI 519, COLO 320 Source : Applied Cell Biology Research Institute and Health Science Research Resources Bank, respectively. Sample Type : - No. of Sample : - Stage : - | Immunoprecipitation and immunoblotting, Nuclear/cytosolic extracts and in vitro kinase assay, Immuno | Phosphorylation | - | Phosphorylation at linker and c- terminal. The linker phosphorylation of Smad2 and Smad3 may represent a target for intervention in human metastatic cancer. |
| 19541409    | 2010 | Cell line : HCT 116 Source : - Sample Type : - No. of Sample : - Stage : - | - | Phosphorylation | - | - |
| 21071512    | 2010 | Cell line : SW 620, SW 480, and HT 29 Source : Department of Pathology, Southern Hospital (Guangzhou, China). Sample Type : - No. of Sample : 168 Samples included 30 cases of normal colorectal mucosa, 93 cases of CRC tissues, 45 cases of metastatic lymph nodes, and 10 cases of metastatic livers Stage : TNM Stage I-IV | Immunohistochemistry, Real timeRT- PCR assays, Western blotting, In vitro invasion assay | Phosphorylation | - | A novel EMT and tumor promoting function for FMNL2, which is involved in TGF-B-induced EMT and colorectal carcinoma cell invasion via Smad3 effectors, or in collaboration with MAPK/MEK pathway. |
Gene Details

| Gene Type | protein coding |
|---|---|
| Gene Family | SMADs |
| Description | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]   | Chromosome | 15 |
| Location | 15q22.33 |
| Start | 67065627   |
| End | 67195195   |
| Strand | -   |
| Gene Sequence |    Download   Select all |
| Length | 129569   |
| OMIM ID | 603109    |
| HGNC ID | HGNC:6769    |
| Unigene ID | Hs.727986    |
| Ensembl ID | ENSG00000166949    |
| PharmGKB ID | -    |
mRNA Information
| mRNA ID | XM_011521559.1 |
|---|---|
| Exon | join(1..734,99269..99462,101153..101227,104928..104978, 115615..115827,119101..119238,121739..121883, 124787..129569)   | mRNA Sequence |    Download   Select all |
| Length | 6256   |
CDS Information
| Location | join(529..734,99269..99462,99627..99758,101153..101227, 104928..104978,115615..115827,119101..119238, 121739..121883,124787..124910) |
|---|---|
| CDS Sequence |    Download   Select all |
| CCDS ID | CCDS10222.1 |    
Protein Information
| Protein ID | NP_005893.1 |
|---|---|
| Protein Name | SMAD family member 3 |
| Molecular Wt. (Da) | 47950 |
| Protein Sequence |    Download   Select all |
| Length | 425   |
| Uniprot ID | P24941 |    
| Ensembl Protein ID | ENSP00000266970   |    
Post-translational Modification Information (Source : HPRD)
| Residue | Type | site |
|---|---|---|
| S | Phosphorylation | 208 |
| S | Phosphorylation | 213 |
| S | Phosphorylation | 422 |
| S | Phosphorylation | 423 |
| S | Phosphorylation | 425 |
| S | Phosphorylation | 208 |
| S | Phosphorylation | 204 |
| T | Phosphorylation | 179 |
| S | Phosphorylation | 213 |
| T | Phosphorylation | 8 |
| T | Phosphorylation | 8 |
| T | Phosphorylation | 179 |
| T | Phosphorylation | 179 |
| S | Phosphorylation | 213 |
| S | Phosphorylation | 213 |
| S | Phosphorylation | 204 |
| S | Phosphorylation | 204 |
| S | Phosphorylation | 208 |
| S | Phosphorylation | 208 |
| S | Phosphorylation | 416 |
| S | Acetylation | 2 |
| K | Acetylation | 378 |
Enzyme Information
No matching records could be found.
PPi Information
(Click to view network : Source STRING)      View   
| Interactant Gene ID (Source: HPRD) | Gene Symbol | HPRD ID | Ref Seq ID | Technique | References |
|---|---|---|---|---|---|
| 60 |    ACTB |    32 |    NP_001092.1 |    in vitro |    15527767    |
| 288 |    ANK3 |    2715 |    NP_066267.2 |    yeast 2-hybrid |    15231748    |
| 367 |    AR |    2437 |    NP_000035.2 |    in vitro;in vivo |    11280774    |
| 675 |    BRCA2 |    2554 |    NP_000050.2 |    in vitro;in vivo |    12165866    |
| 818 |    CAMK2G |    3672 |    NP_751911.1 |    in vivo |    11027280    |
| 860 |    RUNX2 |    2566 |    NP_001019801.3 |    in vitro;in vivo |    10962029    |
| 864 |    RUNX3 |    2565 |    AAH13362.1 |    in vitro;in vivo |    15138260    |
| 996 |    CDC27 |    304 |    NP_001107563.1 |    in vivo |    11691834    |
| 1051 |    CEBPB |    1801 |    NP_005185.2 |    in vitro |    12524424    |
| 1386 |    ATF2 |    443 |    NP_001871.2 |    in vitro;in vivo;yeast 2-hybrid |    10085140    |
| 1443 |    CSH2 |    6688 |    NP_066271.1 |    yeast 2-hybrid |    15231748    |
| 1499 |    CTNNB1 |    286 |    NP_001895.1 |    in vitro |    12000714    |
| 1583 |    CYP11A1 |    326 |    NP_000772.2 |    yeast 2-hybrid |    15231748    |
| 1601 |    DAB2 |    3139 |    NP_001334.2 |    in vitro;in vivo |    11387212    |
| 1655 |    DDX5 |    1615 |    NP_004387.1 |    in vitro;in vivo;yeast 2-hybrid |    15464984    |
| 1874 |    E2F4 |    2806 |    NP_001941.2 |    in vivo |    12150994    |
| 2034 |    EPAS1 |    6787 |    NP_001421.2 |    yeast 2-hybrid |    15231748    |
| 2122 |    MECOM |    1310 |    NP_001098547.3 |    in vitro;in vivo |    9665135    |
| 2308 |    FOXO1 |    645 |    NP_002006.2 |    in vitro;in vivo |    15084259    |
| 2309 |    FOXO3 |    4061 |    NP_001446.1 |    in vitro;in vivo |    15084259    |
| 2316 |    FLNA |    2060 |    NP_001104026.1 |    yeast 2-hybrid |    15231748    |
| 2737 |    GLI3 |    1313 |    P10071 |    in vitro |    9843199    |
| 2908 |    NR3C1 |    679 |    NP_001019265.1 |    in vitro;in vivo |    1   290   233   810   518   520    |
| 3096 |    HIVEP1 |    1925 |    NP_002105.2 |    yeast 2-hybrid |    15231748    |
| 3172 |    HNF4A |    2612 |    CAA89989.1 |    in vitro;in vivo |    10995777    |
| 3308 |    HSPA4 |    9025 |    NP_002145.3 |    in vivo |    12963786    |
| 3725 |    JUN |    1302 |    NP_002219.1 |    in vitro;in vivo |    9732876    |
| 3726 |    JUNB |    1303 |    NP_002220.1 |    in vitro;in vivo |    10903323    |
| 3837 |    KPNB1 |    4114 |    NP_002256.2 |    in vitro |    10846168    |
| 4087 |    SMAD2 |    3221 |    NP_005892.1 |    in vivo |    9311995    |
| 4089 |    SMAD4 |    2995 |    NP_005350.1 |    in vitro;in vivo;yeast 2-hybrid |    9   311   995   109   957   770   000   000   000   000   000    |
| 4093 |    SMAD9 |    4484 |    BAA21128.1 |    yeast 2-hybrid |    15231748    |
| 4149 |    MAX |    1113 |    NP_002373.3 |    in vitro |    12551947    |
| 4194 |    MDM4 |    4082 |    NP_002384.2 |    in vitro;in vivo |    12483531    |
| 4221 |    MEN1 |    564 |    NP_000235.2 |    in vitro |    11274402    |
| 4303 |    FOXO4 |    2070 |    NP_005929.2 |    in vitro;in vivo |    15084259    |
| 4609 |    MYC |    1818 |    NP_002458.2 |    in vitro;in vivo;yeast 2-hybrid |    11804592    |
| 4654 |    MYOD1 |    1166 |    NP_002469.2 |    in vitro;in vivo |    11711431    |
| 4851 |    NOTCH1 |    1827 |    NP_060087.3 |    in vitro;in vivo |    14638857    |
| 5069 |    PAPPA |    1448 |    NP_002572.2 |    yeast 2-hybrid |    15231748    |
| 5097 |    PCDH1 |    4692 |    NP_115796.2 |    yeast 2-hybrid |    15231748    |
| 5324 |    PLAG1 |    4321 |    NP_002646.2 |    yeast 2-hybrid |    15231748    |
| 5325 |    PLAGL1 |    16010 |    NP_006709.2 |    yeast 2-hybrid |    15231748    |
| 5599 |    MAPK8 |    3100 |    NP_620637.1 |    in vitro;in vivo |    10601313    |
| 5657 |    PRTN3 |    1512 |    NP_002768.3 |    in vitro |    15527767    |
| 5717 |    PSMD11 |    5119 |    NP_002806.2 |    yeast 2-hybrid |    15231748    |
| 5933 |    RBL1 |    312 |    NP_002886.2 |    in vitro;in vivo |    12150994    |
| 6175 |    RPLP0 |    1610 |    NP_444505.1 |    yeast 2-hybrid |    15231748    |
| 6497 |    SKI |    1271 |    NP_003027.1 |    in vivo |    12857746    |
| 6498 |    SKIL |    1319 |    NP_005405.2 |    in vivo;yeast 2-hybrid |    1   053   106   215   231   740    |
| 6625 |    SNRNP70 |    1617 |    NP_003080.2 |    yeast 2-hybrid |    15231748    |
| 6711 |    SPTBN1 |    1683 |    NP_003119.2 |    in vitro;in vivo |    12543979    |
| 6721 |    SREBF2 |    2726 |    NP_004590.2 |    in vitro |    15527767    |
| 6935 |    ZEB1 |    1798 |    NP_110378.3 |    in vitro;in vivo |    12743038    |
| 7030 |    TFE3 |    2439 |    NP_006512.2 |    in vitro |    105   572   851   255   194   000   000   000    |
| 7046 |    TGFBR1 |    1822 |    NP_004603.1 |    in vivo |    9311995    |
| 7048 |    TGFBR2 |    1823 |    NP_001020018.1 |    in vitro |    8774881    |
| 7052 |    TGM2 |    1825 |    NP_004604.2 |    yeast 2-hybrid |    15231748    |
| 7170 |    TPM3 |    1840 |    NP_689476.2 |    in vitro |    15527767    |
| 7431 |    VIM |    1899 |    NP_003371.2 |    in vitro |    15527767    |
| 7465 |    WEE1 |    1907 |    NP_003381.1 |    in vivo |    15761153    |
| 7554 |    ZNF8 |    1919 |    NP_066575.2 |    in vitro;in vivo;yeast 2-hybrid |    12370310    |
| 7704 |    ZBTB16 |    11762 |    NP_005997.2 |    yeast 2-hybrid |    15231748    |
| 7750 |    ZMYM2 |    3745 |    NP_003444.1 |    in vitro;yeast 2-hybrid |    14651998    |
| 7849 |    PAX8 |    1335 |    NP_003457.1 |    in vitro;in vivo |    14623893    |
| 8313 |    AXIN2 |    4935 |    NP_004646.3 |    in vivo |    11438668    |
| 8454 |    CUL1 |    4389 |    NP_003583.2 |    in vivo |    11359933    |
| 8850 |    KAT2B |    6780 |    NP_003875.3 |    in vitro;in vivo |    11058129    |
| 8878 |    SQSTM1 |    3319 |    NP_003891.1 |    in vivo |    15761153    |
| 8888 |    MCM3AP |    4483 |    NP_003897.2 |    yeast 2-hybrid |    15231748    |
| 8945 |    BTRC |    4596 |    NP_378663.1 |    in vivo |    11359933    |
| 9146 |    HGS |    5085 |    NP_004703.1 |    in vitro;in vivo |    11094085    |
| 9972 |    NUP153 |    4899 |    NP_005115.2 |    in vitro |    12917407    |
| 9978 |    RBX1 |    6794 |    NP_055063.1 |    in vitro;in vivo;yeast 2-hybrid |    11359933    |
| 10048 |    RANBP9 |    4835 |    NP_005484.2 |    yeast 2-hybrid |    15231748    |
| 10148 |    EBI3 |    12051 |    NP_005746.2 |    yeast 2-hybrid |    15231748    |
| 10401 |    PIAS3 |    9068 |    NP_006090.2 |    in vivo |    14691252    |
| 10428 |    CFDP1 |    16282 |    NP_006315.1 |    yeast 2-hybrid |    15231748    |
| 10610 |    ST6GALNAC2 |    10231 |    NP_006447.2 |    yeast 2-hybrid |    15231748    |
| 10664 |    CTCF |    5005 |    NP_006556.1 |    in vitro |    12099698    |
| 10691 |    GMEB1 |    16059 |    NP_006573.2 |    yeast 2-hybrid |    15231748    |
| 11018 |    TMED1 |    5653 |    NP_006849.1 |    yeast 2-hybrid |    15231748    |
| 11108 |    PRDM4 |    10425 |    NP_036538.3 |    yeast 2-hybrid |    15231748    |
| 22823 |    MTF2 |    14342 |    NP_031384.1 |    yeast 2-hybrid |    15231748    |
| 22938 |    SNW1 |    4340 |    NP_036377.1 |    in vitro;yeast 2-hybrid |    11278756    |
| 23054 |    NCOA6 |    5599 |    NP_054790.2 |    yeast 2-hybrid |    15231748    |
| 23462 |    HEY1 |    4260 |    NP_001035798.1 |    yeast 2-hybrid |    15231748    |
| 51176 |    LEF1 |    1075 |    NP_057353.1 |    in vitro;in vivo |    10890911    |
| 55146 |    ZDHHC4 |    15719 |    NP_060576.1 |    in vitro |    15527767    |
| 55769 |    ZNF83 |    1935 |    NP_060770.3 |    yeast 2-hybrid |    15231748    |
| 56288 |    PARD3 |    5994 |    NP_062565.2 |    in vitro;yeast 2-hybrid |    12650946    |
| 57154 |    SMURF1 |    6902 |    NP_065162.1 |    in vivo |    15761153    |
| 64115 |    C10orf54 |    15160 |    AAH20568.1 |    yeast 2-hybrid |    15231748    |
| 79595 |    SAP130 |    18015 |    NP_078821.2 |    yeast 2-hybrid |    15231748    |
| 79673 |    ZNF329 |    7779 |    NP_078896.3 |    yeast 2-hybrid |    15231748    |
| 79987 |    SVEP1 |    12942 |    NP_699197.3 |    yeast 2-hybrid |    15231748    |
Pathway Information
| Pathway Name | Pathway ID |
|---|---|
| Adherens junction |    hsa04520 |    
| AGE-RAGE signaling pathway in diabetic complications |    hsa04933 |    
| Cell cycle |    hsa04110 |    
| Chagas disease (American trypanosomiasis) |    hsa05142 |    
| Chronic myeloid leukemia |    hsa05220 |    
| Colorectal cancer |    hsa05210 |    
| Endocytosis |    hsa04144 |    
| FoxO signaling pathway |    hsa04068 |    
| Hepatitis B |    hsa05161 |    
| Hippo signaling pathway |    hsa04390 |    
| HTLV-I infection |    hsa05166 |    
| Inflammatory bowel disease (IBD) |    hsa05321 |    
| Pancreatic cancer |    hsa05212 |    
| Pathways in cancer |    hsa05200 |    
| Signaling pathways regulating pluripotency of stem cells |    hsa04550 |    
| TGF-beta signaling pathway |    hsa04350 |    
| Wnt signaling pathway |    hsa04310 |    
Structure Information
| PDB ID | 1MHD |
|---|---|
| Resolution | 2.8 |
| Method | X-RAY DIFFRACTION |
| Chains | A,B,C   |
| Molecule | DNA,SMAD3   |
| Model | View |
Domain Information
| ID | Location | Description |
|---|---|---|
| cd10985 | 224-414  | MH2_SMAD_2_3 |
| cl00056 | 224-414  | MH2 superfamily |
| cd10491 | 8-132  | MH1_SMAD_2_3 |
| cl00055 | 8-132  | MH1 superfamily |
| cd10495 | 232-412  | MH2_R-SMAD |
| cd10497 | 226-425  | MH2_SMAD_1_5_9 |
| pfam03166 | 230-403  | MH2 |
| smart00524 | 231-401  | DWB |
| cd00050 | 232-401  | MH2 |
| cd10488 | 10-132  | MH1_R-SMAD |
| cd00049 | 10-132  | MH1 |
| cd10498 | 229-411  | MH2_SMAD_4 |
| cd10490 | 7-132  | MH1_SMAD_1_5_9 |
| smart00523 | 26-134  | DWA |
| pfam03165 | 45-131  | MH1 |
| cd10492 | 47-130  | MH1_SMAD_4 |
| cd10496 | 232-401  | MH2_I-SMAD |
| cd10499 | 228-401  | MH2_SMAD_6 |
| cd10500 | 228-401  | MH2_SMAD_7 |
| cd10489 | 62-130  | MH1_SMAD_6_7 |
| cd10493 | 46-134  | MH1_SMAD_6 |
| cd10494 | 47-133  | MH1_SMAD_7 |
Ontology Information
| HPRD ID | 298 |    
|---|---|
| Molecular Class | Transcription regulatory protein |
| Molecular Function | Kinase regulator activity (GO:0019207) |
| Biological Process | Regulation of cell cycle (GO:0000074)   |
Drug Information
No matching records could be found.
External Links
| ArrayExpress | GO |    
|---|---|
| ICGC | GO |    
| Cancer3D | GO |    
| COSMIC | GO |    
| NCG | GO |    
| TSGene | GO |    
| Tumor Gene Family of Databases | GO |    
| CCDB | GO |    
| G2SBC | GO |    
| Osteosarcoma Database | GO |    
| RCDB | GO |    
