CoReCG

Search results:

General Information

Gene_Symbol	Gene_ID	Gene_Name	Chromosome	Alias
PKM	5315	pyruvate kinase, muscle	15	PK3; TCB; OIP3; PKM2; CTHBP; THBP1

Evidence
Gene
mRNA
CDS
Protein
PTM
Enzyme
PPi
Pathway
Structural
Domain
Ontology
SNP
Drug
External Links

Evidence Information

Pubmed ID	Year	Sample Information	Techniques	Mechanism	Fold Change	Remark
20812987	2010	Cell line : - Source : Nanfang Hospital tumour tissue bank. Sample Type : - No. of Sample : 150 CRC patients Stage : -	2-D Gel electrophoresis, and mass spectrometry,Western blotting, Immunohistochemistry	Down-regulation	-	This gene differentially expressed in between normal colorectal tissue and colorectal cancer tissue experiment done by 2-D Gel eclectrophotesis In total, 150 patients were involved in the study.
22807066	2012	Cell line : - Source : - Sample Type : CRC tumor and normal tissue No. of Sample : 93 CRC, 41 advanced adenomas, 137 adenomas, 47 non-adenomatous polyps, and 158 normal Stage : TNM Stage I-IV	Immunoblotting and Histochemical Staining,Real-Time PCR,Cell Proliferation Assay,Cell Migration Assay.	Up-regulation	-	PKM2 might be a potential therapeutic target for CRC.Expression of PKM2 was increased in CRC and the increased PKM2 expression was associated with later stage and lymph metastasis of the tumors.
24686087	2014	Cell line : - Source : Offenbach Municipal Hospital Sample Type : CRC tumor and normal tissue No. of Sample : 96 study participants (33 patients with colorectal cancer, 21 patients with rectal carcinoma and 42 controls) Stage : -	real time RT-PCR,Western blotting,mutagenesis and cell transfection,Wound healing assay,Cell adhesion assay, Pyruvate kinase assay	Up-regulation	-	PKM2 overexpression promotes CRC cell migration and cell adhesion by regulating STAT3-associated signalling and that PKM2 may serve as a therapeutic target for CRC metastasis.
22737276	2012	Cell line : RKO, SW480, HT29 Source : Department of Surgery, Affiliated Zhongshan Hospital of Fudan University. Sample Type : CRC Tumor and adjescent tissue No. of Sample : - Stage : -	ELISA	Up-regulation	-	Serum M2-PK can be used as a primary screening test in CRC mass screening. It may be a promising non-invasive biomarker for CRC early detection. Average serum M2-PK (U/mL) was 14.75 among stage III and 13.10 among stage I and II CRC patients, about 4 times higher than that among normal people.
17109496	2006	Cell line : DLD1 Source : ATCC Sample Type : - No. of Sample : - Stage : -	ELISA	Up-regulation	-	Fecal tumor M2-PK is an appropriately sensitive tool to pre-select those patients requiring colonoscopy for the further diagnostic confirmation or exclusion of colorectal cancer. Colorectal tumors were accompanied by a highly significant increase (P < 0.001) in fecal tumor M2-PK levels (median: colon carcinoma, 23.1 kU/L; rectal carcinoma, 6.9 kU/L; colorectal carcinoma, 14.7 kU/L), which correlated with Duke's staging and T-classification

Gene Details

Chromosome: 15; NC_000015.9 (72491370..72523727, complement)
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Gene Type	protein coding
Gene Family	-
Description	This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
Chromosome	15
Location	15q22
Start	72199029
End	72231812
Strand	-
Gene Sequence	>gi\|568815583:c72231812-72199029 Homo sapiens chromosome 15, GRCh38.p2 Primary Assembly CAGTCCCTAGTTCTCCCTACTGCCGCTCCAGTGCCAGAGCCCCTCCGAAGGCGGCCAGGACCTCCAACCA CGCACAAGTCTGCAGCTCTCCCCAACTTTCCGTTCAGCTCAGTCTCCGAGGGTGCGCCAGAGCAGACACC CGGAGGAGTGGGGAGTGGCAGGGCGGGGCCGGGAGAATGCTGCCCCGGAACCCATAAATCTGGGCCCTGC CCAGGTAGGCCGGGACAGCTGGGGTGGCCTGGGCCGAGAGCCAAGAAAAGACACCCCATCTGGCAGCCCA ACTTGGCGGCAACAGGTGGCCCGGCGCCCGGGGGTCTGGGAGGAAAGTCGCTCCGGGGGCGGGCCCCGTT GCCCCGCCGCGTCCCCATTGGTCATCAGGTTTCTTAAAATGTGACTCTGAATCTGTGTCCTTCCGCCGCA GAATTTAGTCCCACCGAAAGGGCAACCTGCCCGCGCGTTCCGCCGCCGCCGCCGCGCTTCCTCCTGAAGG TGACTGCGCCCGCGGGGACGCAGGGGGCGGGGCCCGGGTCGCCCGGAGCCGGGATTGGGCAGAGGGCGGG GCGGCGGAGGGATTGCGGCGGCCCGCAGCGGGATAACCTTGAGGCTGAGGCAGTGGCTCCTTGCACAGCA GCTGCACGCGCCGTGGCTCCGGATCTCTTCGTCTTTGCAGCGTAGCCCGAGTCGGTCAGCGCCGGAGGTG AGCGGTGCAGGAGGCTACGCCATCAGTCCCCACCAAGGGCCAGTCGCCCGGCTAGTGCGGAATCCCGGCG CGCCGGCCGGCCCCGGGCACGCAGGCAGGGCGGCGCAGGATCCAGGGCGTCTGGGATGCAGTGGAGCTCA GAGAGAGGAGAACGGCTCCTCACGCCTGGGGCCTGCTCTTCAGAAGTCCCCAGCGCCGTTCCTTCCAGAT CAGGCGGGTCCGCCGGCTCCTTTCCCGCCCCAGCCCTACCCCCTCATTCTGGTCCCATCCTCTTCCTCCT GCCCCAATCCTCAATGCGCCTCCATCCTCGCCTGCCTTCTCTCGGTCCCTCGTGATTGATTCCACCCTTG CTTCCCCTTTCTCCGCGCCGCCTGTTCCGTGCTCGTTTTCCCCTCTTCCTCCTTAAGTCTGGTCCTTCCA CCCCCTCCTCTTCAAGCTGTGCGTGTCCCCTGATTCTAATGCTTTCTGTGTAACTCATTGAAACTGCGTT CTGGTTCCCCTCCCGCGTCCATTCTCCATTCATGCGCGACCGCCCTTCCCGCGCCCCAGTTCCCTCTCGC CGCCCCTCCCCCTGCTTGCTGGTCACGTCCGCTCCCCCGCATCCCCTTCCTCGCCTGGCGTGTCCGCTCC CTCCCTCCCTCTCTGCTCTGGTCGCGCCCGCCCACTTGCTCCGGTCTCCGAGCGCGGTCCCACCCCCCTT TTCCATCACCGCCTCCCAGCTCCCAGCAGGCTCGGGCGGTGCTGAGGCCCCGTGTCCGGGGCGGGCGGGC GGAGGGCTGGGCTGGGTGCCCCGCGCGGCGGGCGGGATGCGGCGGCCCGGGGGCAGCTGGAGACTTACGT AACGTTGGCCTGCCCCGCTGCCGGGAGGCAGGGCGGTTGCCCCTGCGGCGCGGTGCCGTCCCCTGTGGCC GGGATTAGATGGGCGGCCTGCGAGGGCCTGGGAATGGCTCGGGGCCCGAGAGCTCGACCGGCCCTTGCCG GGTGGCCGCCAGGGACCACGCTCCATCTCGCCGCGGCCGGGCTGCACGTAGCGGCCGCGCCCAGGGCCCA CCCCGCTTCACCGGGCGATGGCCTTGGGCCTCGTAACGGGCGGGATAAACCTCTGCAGGCTTTGCTGGGG CCTCCTGGCCCTCGCCCGTTCCGCGCCTCTCCGGCACGTTTCTTTCTTTTCCTTTCTTTTTTCTTTCTTT TTTTTTTTTTTAATTATCCGGCACATTTTTTAACAAATGCGTCCTGATTGTGGAACGCGGAGGCCGCGGG GTGGGGTGGGGATCTGGTTACGGAGGGGGCAGGAAATCTGTCGCGTTCACTGAACGCAAACGGTGTGGGT CAAGGGCTGTTTGGGGGTCAGAGTTAGAGACCAGGATGACTAGACGAGTCATAGCCCACCGAGCTCACAA TCTTAAAATGTATCTCCTGTAATGCTGGGAGTGGGGTACGAGCTTCCTGCTGTGGGAGGGAGGGGGACAG GAAGCCTCGTAAGGTCTCACCAGGTGGCAAGCACACTGGATTAGAAGATGCAGGAAAGCACCTTCCAGTC TGAAGATCATTTAAAGACTCAGTCATGTAAGGGCATCCCTTGGCTTCTTAATCTACGTACTCCAGACCCA GGGCTTGGACCTTTGCTGGTACGGGTTAAAGTGAGGCCGACAGTGAAGGTCCACGTGGAGAGAGACATTG GGAAGTTGTCAAAAAGGCGTTTAGAATCAGGTTTGACTCATTAATTTCCTGAGACTCTGAAGGAGTTCAG CCTCTACACCTCAGTTTCCCCTGGGAAAACTGGGAGTAACATTTTCCTCATGATGGTTGAAAGGATTCAT GCTTACGTGGATGTGGCACACTCAGTAAGTGTCTAATCTCTGGCGAAATACCCAGAAGAAAAGCCTGTGA CTGAATATATTATTTTTGAGCACAATTACTTAGTCCACAATTGAGCATAAGGGCCTTGATCCATTGTCTT CCTACTGTCCCCAAACGCCTACAGCTAATCGTGAGGAATCAGGCTCTCTGAATAACCACGTGTCTCCTGG AAACCAGTTCCCCAAGGGATCTGGTTTAATATTGACAAAGTAACTGATAATGTAACTTTCCAACTCTCTG CTTGGAGCAGATGCTTTTTGGTCATCTGAGATTGGGACCTGTAGACAAAGGCAGGCAAGGAGGGGCTGAG TTAAGCCTTTGGTGTTCCGCTGTAAGTAGAATACTACCTACCAGAGGAACATTCCAGCCCTTGACTTGCT CTGCCTGCTGGTGAGATTAGGATTGGAGCCAGGGTCAGTAGCCCCCAGTTGGCCGTCTTGAGGGTGTGGC CACTCCCTAGGTCACAGAGGAGATGGAGGTCAGGACTCCCCTGCTGCAGCGTTGTTAGCAAATAATTTGC CCTTTGGTGGCCTGAGGTGCTGTGGGGGCAGGAGTGGGCTGTCTTGTGGGGAAGGGAAATGTAACGTATC ACCAAATCTGTGGGGGAGTGGGGCTCTGTGGGGAAGATGACTCAACCTTTCAATTATTCTGCTTTTGAGA GAATTATCTTCGCTGGGTGAGTGGGGAAACTACATTGTGGTTCTTGCAGGCTTAAAAAGTCCCTTCTCCC CTTTCAGTGAGTCAGATAATGAAGCATGCATTTCCCCTAATTAAAAATGCCTCAACTACGGAGCTTGCAG TGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCTTCTCAAAAAAAAAAAAA AAAAAAGCCACAACTAATTAAAAAATGATTGGTCCGGGTCACCTGGTTCTCTGTACTGCCTTAAGAGCTT AAGTGTAGACTGGCAGAGCCCGGTGCCCAGCTAATGCCGTCTGTGCAGCTTCATTCCTGGCCCTTTTGTA GCGGGCACAGCCGCCTCTCCTGGGACTTGATTAATGATGACTTAAAGAAGGTTCTTGAACTCATTTCTCA GTTACTGACTTGAGCCAAAATGTATGGATGTTGGTCTCCTGTCTAGCACAACTGCTTTCATTTTAAGCAT TGCATTAGAAATAATGTTGTATTCATATTTTAGCAAAGAGCATCATTAGCTCTTTAAGAACCGTACCCCC AGTGACTTAGCAAATTTGGTGGCGGTGACTCTAAACAGCTAGCACTTTATGGGAGTTGCTTCTGTGCCTT GAATGTTGTGACACTGATGTGGGGTCACAGGAAAAACCATTTTATTTCCTAAAGCTGTTTATTTCTTAAT ATTGAGGCAATGGAAGAAATGAGGAAACGGCCGTGGGCAGGAAGGTAGAAGAAAATGCAGCACTTACATT ACAAACTCACTTTACTTGGGTTTTAGTTTATTTTGCTTCAGTTTTTTGTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTGAGATAGTTTTGCTCTTGTTGCCCAGTCTGGAGTGCAATGGTGTGATCTCGGCTCACTGCAA CCTCCACCTCCTGGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAAGTAGCTGGAATTACAGGCACTTG CACCACGCCCAGCTAATATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGCTGGTCT TGAACTCCTGACTTAGGTGATCCACCGGCCTCGGCCTCCCAAAGTGCCGGGATTACGGGCGTGAGCCACT GCACCTGGCCTGCTTCAGCTTTTATGAAGGCAAGTCAGGATGTGTTATCTTGGCCAAGAGCTAAGACTGA GACCGGAGTAGAGGAAAATGACTCATTTGACATTACCCTTCTTTCTGAATCACTCCAAAAGTCCACAGTC AAATCTCAATTAGCTGGTATAACACAAGAATGCGTTGACCCTGTGAGCAGAGTTTTATTGCATCTTCAAG GGTCTTCGTGAGATTGGTTTATTTGGTATGAGGTCTTAAGGATTTTAAGTTGTCCCTTTAAGCATCAGTT CTGATACAGATGGGAATAGAGCTAAACAACCAGATATATGAAGACCATTTTTGATACCACCATACTTGCG GGGAGCTTGCATTTTTTTACCAAGGGGCAGACTGGATAATTGAGTAATTTAAATACTTGGGACTTTGTTA AAGAAGCTGTTGGGGGAAGGTGAGCCTGGGTGAGTGGTGGAGGGTGATGTGCAGCATCTGCTGGGCCTTC CTGCCTCTTGGTATGACTGCTCAGCTCAGGAGTTGGGTGCTTTAGGGCCCCTACCATGCAGTGCGGTGAA GCCCCGCCCCTGCAGTGGTTTGTGCTTGTCTGCACGTAGGAGGGAACAAGCCGGCTGCAGTACCTTCGGT GGCCTCTGAGACTCACCTCCCCCACTTCTGTCCATAGACAAAGCTCCTGGGGAGCCCTAAGCCTCTTTCC TCATGCCAGCAAGCAGTCTTGGAAGCAGGCTGGGGCTGCAGTGGGAGAGATGCCAAGACCAGTTATTCAA GGGCAGTGAGGTTGCCAGTCCTAGCATTTCACACTGTGGACCAGCACAGCTGCTGGCTGAATACTGCAGT CAAGATTCTCTGAAGGGAGTCCTATCCCTTCCAAACATCTTCAGTTTACTTGCAGATAATGTTTCTTCAT TTTTTATTTTATTTTATTTTATTTTATTTTTTTGAGACGGAGTCTCGCACCATGACCCAGGCTGGAGTGC AGTGGTGCAATCTCAGCTCACTGCAAGCTCCGCCTCCCGGGTTCATGCCATTCTCCTGCCTCAGCCTCCT GAGTAGCTGGGACTACAGGCAACCGCAACCACGCCTGGCTGATTTTTTTTGTATTTTTTAGTAGAGACGG GGTTTCACTGTGTTAGCCAGGATGGTCTCAGTCTGCTGACCTCGTGATCCACCTGTCTTGGCCTCCCAAA GTGCTGGGATTACAGGCATCAGCCACCGCGCCTGGCCATGTTTCTTCAATTTTAAACAACTGATATCTCC CTTGGCCATGAACGAAAAAGAACTGCCCATCAGTGGAGTCAGTCAGGGCACATAAGACACACTGTGTCCA CCATGCCATTTCAGAGGAGATTTGATTGAGTTAAGCAGGGAAATAGAGATGTTGTAAACGTTGAAACTAT CTGGGTATCCCTCTTTGGTTATTAACATTAGATGAGCAGAAAAACAAATGTCACCGATGGGCAAACATTT AAAAAGTCTGGCAGTACCAAGTGTGGAAAAAGGTGTGGAAGGCAGCAACTCAGCGTTCATTGATATAGCC ACTTTGGAGAGCAATTCAGCCTTATTTAGTAAAAATAGAAATAAACATACTCCATGACCTAAGACTTCAA TTTCTGGATATATAGAAACTCACACAGTACAGGGAGACATGTACTACAAGAGTATTGATTAATAGAAGAA ACTTAGTTTAACGGGGAGGGAGAGTGGCCACCAGTAAGACAGTCCATAAATAAAATGGTCTGTTGTACAG TGGACTAGTGTAACAGCTTAAATGAATTAGCTAGATCCATATACCCACTGGATGGATCTTAAATGTGCTG CTGAGTGAAAAACAAGTTACTCAGTGATATATACAGTATACCACTTAGGGCATTAAGAAAACCACAATAT TATAGGGTTCAGTATAGACATAGATACACTGTACATAGACTTCAGTGAACTGGAAGGATATAGAGTTCAT GACAGTGATTGGGTGTCAAAATGCATGATGTGGCTGGGAGCAGTGGCTCACACCTGTAATCCCAGCACTT TGAGAGGCTGATGCAGGAGGATCACTTGAGGCCAGGAGGTCGAGACCAGCCTGGGCAACATCGCAAGATT CCCATCTCTATTTAAATAAATAAAATAGAAAAAAAAGTTTAAGATGGAGGTGGAAAGGGTTGGAGGAGCT TGGGGATGGAGAAAAAATGAACTGTATAAAATTAAAATTCTTGTTTTTGTTTTTAGAAAGTAAAGAGGGC CGGGCACAGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAAGCTGAGGTGGGCGGATCACGAGGTCAGG AGATTGAGACCATCCTGGCTAACACGGTGAAACCCCGTCTGTACTAAAAATACAAAAAAAAAAAAAAAAA AAAATTAGCCGGGCCTGGTGGCGGGCGCCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATGGC CTGAACCTGGGAGGTGGAGCTTGCAGTGAGCTGAGATCGCGCCAGTGCACTCCAGCCTGGGCAACAGAGC GAGACTCCATCTCAAAAAAAAAAAAAAAAAGAAAAAAGAAATTAAAGAAAATACAGCTCAGCCTTTATTT GTGTTTTTTTTTTTTTCCTTTTTTTCTGAGACAGAGTTTTTCACTCTGTTGCCCGGGCTGGAGGGCAGTG GTGCGATCTCCGTTCACTGCAGCCTCCACTTCCTGGATTCAAGCAATTCTGTGTCTCAGCCACCCAAGTA GCTGGGATTACAGGTGCGCGCCTGGCTAATTTTTGTATGTTTAGTAGTGATGGTGTTTCACCATATTAGC CAGGCTGGTCTCGAACTCTTAGCCTCAAGTGATCTGCCCGCCTCAGCCTTTCAAACTGTTGGGATTACAG GCGTGAGCCAACACAGCCAGCCATGGCTCAGTGTTAATGGTCAGTTCTGGGTGGTAGAGAGGCAGATGTT AAAACTTTTTTTTCTTTAATTCGTAACATAGAAGCAAACCTATAAAGGCTGCCGTAGGAAGACCAGTCAT AGTAACTAGTTCAGTGCTCTTGGAGAGTTGGCACTGCCTTTCCTCCTTTATCCCCCCGACTAGAATGCAG GGCAGCCCTTCCAGTAAATGTTGAGCCAGTGCCTCACTTTGCTGAGGCCATCACCCACCTTAGTTGCACT TAAGAGGACCCTAAATCAGGGTCCCAGGTCCCTTGCTGATTTTAGAGTGTGGATATCATACCCAGAAACA CCGCCCTACTTTTAATCCTAGTAAGGAGGCACCATGTCCCAGGACAACTAATGCTTCCCCCAAACCACCT CCTTCAGGCTGAAACCAGTTCTCTGCACTGAGCAGCTGGGATGGAACCAGGAAATCCTCGGCATCTGAGG ACATTGAGGGGTCTCTGACTTAGGGCTTCTTCACCTGAAGTTGAGTGGTCTTTGAGGGAAGTAGGCCCAT TTAGCATCAGCTGCTCTTCCCTATTCCACACTCTAGTTGGAAATAGGACCTTAGGTTCCTGTTGACAAGT CATTTACTTTCAGCCCCGAAGAAATAAAAGAGCCAAGATTTTTTTTTTTTTTTTAAAGCCAGGGAATTTT ACTAGAACCTACAAGTGGGCTCATTTTGTTCTGTGTAGCCTGGTAACACCATACTGCTTTCTGCTGTGGG GCCTCCTGGGGTTAAAGTGTGGGCTTAAGACCCAGGTCTCTTAGCTAGAAGATATCTTATCCTCTGTATC CTGCACCCATATGCAAATACATTATTGTCATTACCCTTAACTATAGATGAAGATGAACAGTGCCTATTCC AGACCTTACTAGGTTCTGCTGGCCCGTCACCCATTTTGATCATGTTGCTGGCCTAGTTTGATTAGGGCAA ATCTTAGAAACTCCATTTCCATTGTTGAGGAAGAGAACTAGAGAGCAGGCTGACCTGAATGCCAGCGTAT CATGATGCAGACTTTCTAACGGATGCAGGTGTTCGGAAGAGTTGTGGATCGAAACGCCTTCATGATGGCT TGGAGGTGTAGGTAGCAAACTGACGTCACAGGAAGGAACACAATCTTGGGTACCTACTGGCAACGTTGGA GGGAGAAAGTGAGCATCAGGTGCCATCATTTTATAGTTGATCTATGTGATGAGGTTGGTATCGGAGCATA ATTGGTACAAAGGAAAAATGACTTAGGCAGATGCAGACTCACGGGCCAGGCTATTTTATTAGGGCAGAAT GATTTGGTCCTTTGTGGAAGAATTGGTGGAGTGAAGCGTGAATCTTTCCCAGCACAACCCAACAACAGTC CTGGCCCTAAGAAGTGGAGCATGGGAGTTGGGTGTGGTTCGTGCCTGTAGTCCCAGCTACTTGGGATGCT GAGGCTGGAGGATCGTTTGAGGGTGCAGTGAGCTATAATATAATATAATCACACCACTGCACTCCAGCCT GGGTGACAGAGTGAAACCCTGTCTGAAAAAAAAAAAAAAGAAAAAAAAAAAGTTGGAGGGAGGAGTGTTG GGTATTTTTTATGATTTTTGTCTCCTGGTTTCTGAAGAATGGCCAAAAAATTGTGTCTGACACAAAGGAA ACTAATATAAAAAGCCAGGAGCTGTCTGAGATGAGAAAGGAAAGGGAGAATAGGGCACTTGGAGCTGAGC TGTGATTGTGCCTGTTCCAACCTGTCACTCCAGACTAAGGCCTCTTAGAGATGGTGTCTCCTTTCTCACA GAGGAGACATGGCTCTGAGGAAGATCTTACTGCAGGGGCTCGGGCTCAAGAATAAGGCTCCTGGACCTGG GCATGGTGTGTGCTGCTATCAGTGGATACGCCAGGCTTCCACTGCTGGCTGGAGGTTGGCTCTGCATGTC TGTGCCTTCCTAGGAGGAGGATGCAATAGTGAGTCAGACTGGCATGGGTGGGGCCACATGTCCTGGCAGG CACTGTCCAGGCAGCTGGCATGAGGGAGAGGAGTCTTTCCCAGAAGTCCGCCCTGAGAAACCAAGGCTGG GCTGCTCTCCTGGAGCCAGGGGAGTCCAGTGAGTGTTTTACATACAACTCTAGGTACGTGGTTGGTGGGA TGGGCAGTTTGTGCTGGGAAGAGGCTTGTGGAGGATTTTGGAGAAGGCAGGAGAGCTCTGGCCCTCCCTG CAAGGGAGGCTTCAGGTCAGAGCCTGAGGAAAGACCTGGGCATGAGATATGAGGTCTCTGGCTGGGTAGA GAGCATGAAGGACACATGAGATCTGGAGTCTGGATGAACTTGCTGACAAGCAAGCTTTTTTTGACTGCTA ATGCGTAAATCCACTATAGAAATTTTCATTTGTCATTTTTGTACTTATTGGCAAAAAATTAGAGCTTATA GGCATCAGATTTAATTTGAGATAGTTGAAATAGGGTTTGTGTTTGAGGCCAATATAATTTTATCTGCTAA TGGCATCTCGTGGACTTGGAGGCAGCCCTTCTGTACCAGAACATTGTCAAAAGCTTTTACTGTAAAGCTT GAGAACAAACTAGTTTGCTGGATTTGGGCATGTAACTAACAGGTTTGAGGCATGGGATTATCCTGTGGAC TTTTTTTTTTTTTTTTTTGCTTTGAGGTTCTGAATGTATTAAGGTTGACCTTCTTAAGCCGTTGAAACTG TTCTGCAGTACATTTGTGATGTAGGGCCTAAGACTTGTATCGTTTTTTTTTTTTAATCACAACCCAGTGT ACAGAACTTGAGACATGCGTCTTTTCTCTGCCACCTTTTAAAAGCAGATTATTCTTGAAGTGCATAGAGC AGCAATTGATTAATGGAATTGGTGTCTTCACATTTCATTTACTTCCTCCCAACAATTTTATAGGATGCAT ATAAATATTTCCAAAAGAGGTACACATAATTTCTTACTATAAAAGTATTTTTATATTTATATCAGTAAAT TTGTTAATAAAGAGGATTTTTTTTTTTCTGTAATCCTACCACCCCAATGACGTCCTATTAAAATTTCAGT ATATATCCTCCCAGGTCTTTTAGGTATGTTTAATTTGGTGTCCCTTCCCCGCTCCCAAAAGGGGAGGGAC CAGGTTCTTGTATAGAATAGTGGAATGTTAGTAAATCACAGGTTTAAAGAGACATAACAGTGGAATCTCT AGAGCAGCTGTCACCTGGATACCTGGTTATTAAGGTAATTTTTCCATTACCCCAAAGAGCTTTAGTTACA CTCAGCTTTTTCCTTAATCCTTGTGCAGCTCTCCAGGGCACACCGTATTCAGCTCTGAGCGGTCTTTGCT AGTGAGGCCAAGGAGCCACCCTGAGCCAAAAGGGGAGCATTATGTCACCGGAAGCCCAACCCCAGAGAAC CAAAGGTATGACCTGATATTCAGTGGCCCCAGCCAGGTCTTTACAGGAAGACCCTCATATCTCAGGTCTA AGAAGAGCCAGCTGATGGTTTTTAAAAAGAGTGGAATTAGTTACTCCAACCCACTTATTCAGATCTTATT TTGTTCACAATACAGTCCCTAGATTGTAGGCCCATTGGAGGCCACAGCAAAGCCTTTGTGTTCCAGTTGG CCTGATGTGCCATCTCTCAGTAATGTTCCCTTAACAGCCAGACTTCCCTAAGCCCAGCTGGGAGCTCTGA AGGTATGCGAGCCCTCCCTCAACCATGAGTGTAGGGAAAGGGACCAGGGGCCCCAGGCTTTCCTGTCAGT AATGCAGAAGTTCCTCAGATTTAGGGAAGGGGGAGCAGAGGCATAACTTTGATTCTGACAAAGAGGCATT CAGAGAGACTGAAAGGTCATTTAACAAACACTGGAATGCTTCCACATACTAGGTGCTAGGAGATACAAAA CCATATAGGTCCTGGAAGGGAGGATTGATTTTTTCATTTTGGTACGTAGTAGATATTAGGGGCTTAGGAA ATACACATCGAAATGAAGAGTGCATTTGCCATGTTGAACCGTTAGCCGGTATCTTATTTCCCCATTTTAA AAGTTTTAGAATCTGTGGTTGAGGACTTGTGGCCATCAGTTTTCCATAGCCAACAGACTGTTCACTACTG CCTTCAGAGCTCCTTGGACCTCAGCGGGCCTTCTTTGGAGATGGCAGAGATGGATTTAGATGTATACTCT ACTCGAGCCACCCAGAGAGCCCACAAAGTCAGAGATGGAACAGGGTAAAGGAGTAAGGGTCATATGTGTG AGATGCCTTGATTTGGAACTTTGAGATTTAGGATGAGGTGGGGAAGGGCTAAAGAGGAGCTTGTTCCTGA GCCTTGCTTGGCCGAAGCATTTAGGCTCAAGCGTTTTAGAAAGAGTAGCCCTTGGTCTGAGAACTCAAGG AAACAGCTTTCTGATGAGACGTGTAGCAAGCTTCTGGTTCACATCCTTACCTGATAGTTCTTCAAACACT GCCTGGTCTGGTTCACATCCTTACCTGATAGTTATTCAAACACTGCCTGGAAGCTTCTCCTGAGTTTTTG TCTCTAATCAGCTAACTAACAGGCTGAGTGAGTTTAGTTGTAAGTCATTAATGAAGAAAGCAAAGGTTGG GGCCATTGTCAGGGTTGTGACCTGGGCTAGTTAATTACCTGGAACTGATGGTCTGTGTTACAGAGTGGTG GTATACTTGTCAGGCTTAGAAAAGAAATCAGGATGTGTATCAAAAATCATTTGGGGAAAAGATTTGACCA GCAACTTTAATTTCTCTATGTTTGCAACTATCCTGTTAATGTAGTTGTGATAATTTCAGAATTATACCAG TGCCCTTATGTTATCCTTGCTTTGCAAATTGCAAATTGCTTTGCGTGTCCTGACATCCTTCTGGCCAACA GTAGATGTGGTTTTAGGTTTAGACTCCTGGGATGGAAGCTTTTGCATTCAGGGGAATGACTTTGGGTTTG GGTGAGGATTGTAAAGAGGCAATATGGGTGCCCCACGACAAAGCAGCTATTTGTAGCTTTGTGACAGCTT GACATGCAGAGATCTAGGCTTATCAAGGCACTAAGCTAGGAGTCAGTTGTTTGTATCACTGGAAGATTGG TTACAACTTCCTTCATTGGAAGCTCCTTCAGTGCATGTTAAATGATGTTATTTATAGATAGGGTGGTGAG AAAGCTGTCTAGGTAGATGTCAGTCAGCCCAGTGTAAGAGAGACCTGCTTACTGTGGGTGCTTGGGACTA TGTGGAGTGGGTGGGAGGTTTTAACTTGTTCAGTAAGGTCCTTTCCATTGTTCACAATCTGGTGAACCCT TTTTCTAACATGAGGAGCACCCACATAACCAGATCATGTCTGGCTTCCCTGTGGCTTGTGTACAAAGCGT GCTTATTGAGTTAATGTGTAAGCAGGAGACAGCCTTCTGTGCTAAATGGTATATTAACCACTTCTCAGTC TTACCACTCTCTTTCAATTTGTCTCGACCCAGGACCTCAGCAGCCATGTCGAAGCCCCATAGTGAAGCCG GGACTGCCTTCATTCAGACCCAGCAGCTGCACGCAGCCATGGCTGACACATTCCTGGAGCACATGTGCCG CCTGGACATTGATTCACCACCCATCACAGCCCGGAACACTGGCATCATCTGTACCATTGGTGAGTGGGTG TGCCCCTTCCCCCAAAAAAGGGCTTCATGGGCAGTGACCTTTCTCTCCTGAAAAGAGTAACTAAATGTCC TAACAAACCTAGGTGCTACATGGGATACTACACAGATTCTTATGAAAGGACTCAGGTCATAGGAAGTTGC AGTAAAGAATTAGTATGTGCATAGGATGGCAAATACAGTTAATAAGAGAGTATTAGACATTTCAAAATTG CTAAGATGGCGAGGTATGGTGGCTCCCAGCACTTTGGGAGGCCAACGTGGGAGGATTGCCTGAGCCTCGA AATTTGAGACCAGCCTGAGCAACTTAGACCCTGTCTCTCCAAAAAGTGAAAAAAAAAAAAAAAAAATTAG CTGGGCATGGTGGCATGCACCTGTAGTTCTGGCTACATGGGAGGCTGAGACAAAGATCACTTGAGTCCAG GAGATTGAAGTTGCAGTGAGCCATGATCACACCACTGCACTCCAGTCTAGGCAACAGAGCGAGATCCTGT CTTAAGAAAAAAAAATTGTCCGGGCGCAGTGGCACATGCCTGTAATCCAGCACTTCGGGAGGCTGAGGCA GGTGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAATCCCATCTCTACTAAAA ATACAAAAAAATTAGCCGGGGGTGGTGGCGGGTACCTATAATCCCAGCTACTTGGGAGGCTGAGGCAGGA GAATTGCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCTGAGATCTGACCATTGCACTCCAGCCTTGGCA ACAAGAACGAAACTCTGCCTCAAAAAAAAAAGGAAGAAAAAAGAAAAAAACATCGCTAAGAGTAAATTTC AAATGTTCTCACCACAAAAATGTTAAGTATTTGAAGTCATGGATATGTTAACTAACCTGATTTAATTATT CCACATTGTATCCAAACTGTATGTATTGGATTACATAACTTTGTAACCCAAATTATAAATTACCAGTTTA TAATAAAAAATAATTTGTTGCAAAAAGAATCCATATGGTTTAGGTTTTATGCTATAGGCAAAATTTAGAA GATGTTTTCCTTAGCAGGTCTTTGTAGGAGCAACTTAAAGACCTAGGAAAGATCTTTCTAACATGTTCTG TGCTACCAAGATTCTGTGGTTGGACATCTGGCTGGGTTTCAGTGAGGGTGGAGAAGGCTGGCCAAGTCTT AACCTAGGCTTTTCTGATACAGTGGGAGCCTGCAGAACTTGAAGGAAATGGTCGAAGTGTCCCAGTAGAT CAAGAAAGTAAGCTGGCACGGTAGTAGCCTTCCATGCACTTTTTAAAGACTTTTGAGCTATTTGGGAGAG GAAAAGTTTTCAGGGAAAAAAATTCTTTAAACTTAAGCAAACTTAAATGTTTTTCCTTCTTTGAATAATT AATACTTGTGGCTTTAAAACTTTTCCTAATAGGCCCAGCTTCCCGATCAGTGGAGACGTTGAAGGAGATG ATTAAGTCTGGAATGAATGTGGCTCGTCTGAACTTCTCTCATGGAACTCATGAGGTGAGCTGTGGCTGGA CCCTATGGCCATTGTGATGGCCTGTAGGAAACAGGGAGGGGGTGCAGTGTTCGTTTAGCCACAGTGGACT AGACAAGGATGAGTCTGAGTTTCACAGTCAGTGTGAAGTTTGTCTTTACTAGCCCATCCCTACTCTCCTT CCCTCTTGTCCTGACAAAGCAACTGGCTGAGTCTCTTTTAGCAAAAAGGACCCCCTTTGTTGCTGGCTGT GGTTCTCCCACACACCTCTCCTACCCTTAGCTTTTACAAAGGAAGATATGGAAAGGTTCTACTGGAAAAC CCTCTAAGCCTTAGGTGTCCTGGCCACAGCGCTTGACTCTCCTGTCCCAGGGTTTCTGCTTCACCTTGTG TTGCCATGGTAAACCATCTAGCAGATTGATTCTAGCTTAGAACCAAAATAACTGGGCAGGTCCATGAGAA CGGTTTCCACTATTCTAAGTTTTGAGGGACTGAGCCTAATGCATAAGCACTATCTGGGGTGTAATACCCC ACTTCCTCAGCACTGTATTCTCAGCCTGTGCCTTCCCAGGGGTTCTGGTACATTAAAATAACACCAGTTA GCACTCTTCCCCAGGAGCCTAGTAGGACTGTATTTGTGCTGGGCTCTTTATTAGCTGGCTTTACCTATGG ACAGAGGCCTTGCCCAGGAGCCAGGTAGCAGCTGTTGGGATGGCTCCATTCCTGCCTCCATTGCCAGATT TAGAATTAACCCATTCTGAGGAGCTTGGGGTTCCCTGAGGTACCATGACTTATTTATTTTTTTATTTTAT AAAACAAAATTTTGCTCTGTCATCCAGGCTGGACTGCAGTGGTATGATTATGGCTAACTGGATCCTTGAC CTCCCAGGTTCAAGTGATCCTCTTGCCTCAGCCTCCCGAGTAGCTGGGAATACAGGCATGCACCACCACA CCTGGCTAATTTAAAAATTTTTTTGGGGGAAATGAGGTCTCACTATATTGCCTTGGCTGGTCTCAAACTC CTGGGCTCAAGTGATTCTCTCAAATGTTGGGATTACAGGAATGAGCTACCATGCTCAGCCTGGGATTGTG CCTTTTTAAAACCTTCAGACTTAACCATAGGTTTCCCATAGATCATGGGATTTCGTAATGGCATTGATAA GAGGAATTACAGAAGAGGCAAACTTTGCACCTGTCTTGGCTTCTGTATTTCCTGTTGAGAGTAAAGAAAA TGCTATCCTGTAAGGCCAATTGCCTTACAGAGGTTGCCCTCTGGCATTTGGAAGTTGGTATTAAGTTTGG ACTAAAAATAAAGCCTCAGGAAATGCAATCCAAGAGTGAATTCCTCCTTTTGGGAAACACAAGACTCTTC ATCATAGATTCCCTAACCTGTGTTCATAAACAGCCTATGGCCTGGCTAGTGGCTGGCCCTTAAATGTCAT GGGGACCTGACCAAGTCCAGCAGACATACCATGTAGGTTAAGACATGTCCCTGTACCTTTTGGAAAATTC TGTAGTTTTCCAAAAGCAAGGGGTCCTTAGCAGGAGTCACCGAGAATTACTTGTTAGAGAATTAAGTGTT AGCTTAGCTTAGAGAGAGCTGAAGACAATGCTGGAGGTCTGTTCGCTGTTGATCCCTGCTGCTGTAGTCT GCCATGGGCTCCTGCATTCAGGGGAAGGAGCAGAAATAGATTTTTAAGAAGTTGACCTTTAAGTAGGCTT TATGGTTCCTTCATCCAGTAAAATAACACCACATAGCTCTAACATGGCAAGGGCGAGTGATACCTGCCAC ACCTGCTGGATGAGAGCTGGCTCCGATTTTGGTATTTTAAACTTTAAGAGGCTTTTGGAGATTATCTCTA CTTTCACTCCTATTCCCAGATTATAATTAAGATTTATTTTTTATTTTTTATCTATTTATTTTTTAAAGAT GTCCCTCTTGTGTGTTCATTTTGAAGTTTTAGACCAAGATGAGGTTGTGTGTGGGCTCAGCTTGGAAACT GATCTGAAATTATTCTAATTTATATAATGTAATGTAAACAGTTTCAGCCTTACCATACGTCAGGGCTATC GTTTCATGTGCACCTTTGACTAGGGGCTGGGGCGTACTTTTCCAGTTTCTGACTATTTTAAATGCTCTTC TGAGCAGAACGTTGAGATTACTGTCTTCCCTCTCACTCTGACAGAGGGACATCAAATGTCTGCATCTGAT CTTTTAACAGCTTTTTTTTTTTGAGACAGAATCTTGCTCTGTTGCCCAGGCTGGAGTGCACTGGCACAAT CTCTGCTCACCACAACCTCTGCCTCCCAGGTTCAAGCAATTCTCATACCTCAGCCTCCTGAGTAGCTGGG ATTACAGACCTGTGCCACCACGCCCAGCTAATTTTTTTATATTTTTAGTAGAGACGGGGTTTCGCCATGT TGGCCAGGCTGGTCTTGAACTTGTGACCTCAGGTGATCCGACTGCCTCGGCCTCCTAAAGGCGTGAGCCA CCACGCCCAGCCCTCTTTTAACAGCTTTGGCAACTAGTCTTCAGCCCTCACTTTTGGCAGTTCACATGGG CAAGATGCATTCTTGCTGAACATGTGGTTCCATATGCCATGTTTTCCAGATTTATTTATTTATTTATTTA TTTATTTAGAGAGGGAGTCTCGCTCTGTCATCCAGGCTGGAGTGCAGTGGCACGATCTTGGCTCACTGCA ACCTCTGCCTCCTGGGTTCAAGAGATTCTTCTGCCTCAGCCTCCTAAGTATCTGAGATTACAGGCACCTG CCACCACACCCGACTAATTTTTGTATTTTAGTAGAGACTTGGTTTCACCTTGTTGGCCAGGCTGATCTCG AATTCCTGACCTCAAGTGATCCATCCGCCTTGGCCTCCCAAATTGCTGGGATTACAGGCGTGAGCCACCA CACCTGGCCTAGAAATAATGACTTTTAAACAACCTAAATGTAGAGCCTTCCACAGGACAGCATTGATGGA TGCTTTACCACATAACATCCCAATAAAGCCACAGCTGAAGTGGAAGACTCAGTACACCTCCCAGAGATGC TCTAAGAGATTATGATATATGACATAGATTTGAATAATATACCTAATAATTGGTATGTTTATAATATATG GTTTTACATCCCCAAGACCAAAAATGCATGTTTGCATGAAACACTCATGGTTACAAAAATATATTAGGCC ACCAAAAAAACCCCCACGTTTCATAAAGTAGAAATTATACAGACACATTCTCTGATAAAATTTTTTAGTG GAAATTAAGAACAAAGTCAAGAAAACTGAAGTGTGCTTACTTTAGAAAGCAAAGATCTCAAGGTAGATGA AATAAATATTTAACTCAGAACACTAGGGGAGGAAAACCCTAAAAAGGGTGAAGAAAATAATTTTGTAAGA TTATAGCTCAATGAAATGAAAATAAATTTGACAGATTAAGCTAAGAGCTGATTCTTTGTGGGGAAAAAAT AGTAAAATAGAAAGTTCTGAGAAGCCAGGTGAAGAATGCGAGGATGTGCAAATAAGAGTATGAATAGAAA AGAGAATATTTCCTACACATTGGAGATTTTAAAAGTCAAGAAAGACTTATAACTTAATTCCTATATAGAG AGATGACTCTGGCTATTTTCAAAGAAAAGATAAATATCCAAAAGATAGAAAATATGAATAGACCAGTGGC CATAAGAAGTTGAAAAAGTGGGCTGGGCGTGCGGTGGCTCACACCTGCAATCCCAGCACTTTGGGAGGCC AAGGCGGAGGGATCACTTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCTGTCTCTA CTAAAAATACAAAAATTGGCTGGGCATGGTGGCACATGCCTGCAGTCCCAGCTACTCGGGAGCCTGAGGC AGGAGAATCGCTTGAACCTGAGAGGTGGAGGCTACAGTGAGCCAAGATCGCGCCACTGCACTCCAGCCAA AAAGTTGAAAAAGTGATTAAGATCTGGTTCACCTCAGAACACTTAAGTCCAAATGATTTTAGTGGCTGAA TTGTCTCCCCTTCAAAGTTCAGTTACATTGTTAAACTGTTCCAGAGCCTAGAGAAATATAGAAATCTTCC CACTGTGTTCTTTGAAACCAATATACGCTGATACTGAGATCAAACAAGGACAGTACCAAAACCAGGCAGG TACTAACAGTTAGTGTGCTAGACCAGTCTCACTTAGATGCAGAAAACAAATAAAATTTAATAATCCAAAT CCAGTAGTGATTGAAAGGAATGTCTTGATCCATGACCAAGTAGATTTTATTCTAGGAGAACAAAATTCTA CATCGGGATTAAGTAGAGTTAAGGTTGACATTTTTTTTTTTTTCTTCTGAGACGGAGTCTCGCTCTGTCA CCCAGGCTGGAATGCAGTGGCACGATCTCGGCTCACTGCAACCTCTGCCTTCCGGGTTCACACCATTCTC TTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCCTGCTACCACGCCCGGCTAATTTTGTTTTTGTA CTTTTAGTAGAGACGGGGTTTCACCATGTTAGCCAGGATGGTCTCGATCTCCTGACCTTGTGATCCCCCC TCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACTGCGCCTGGCCTGAGTTAAGGTTGACTT TTAAACAACCTAAATATAGCTAAATATAGAGCCTTCCGCAGGACAGCATTGATGTGTGGAACTCTTATCC ACGTGATAACATCCCAACAAAGCCACAGCTGTAGTGGAACTCAGTACACCTGAGTCTTATCATTATAAGA TGATAATAGGTAACATTTATTAGATAATTACCATGTACTTTGTCCTAATACTTCATGTATTCTTTTACTC CTCACGTCAACTCTGAAGGAAAGGCACCACCTATCCCCTTAAAAGAAAACAACTATTACTATTCTTTTTT TTTTTTTTCTTTTAGAGACGGAATCTCACTCTGTCTGTCGCCCAGGCTGGAGTGCAGTGGCACGATCTCG GCTCACTGCAACTTCTGCCTCCTAGGTTCAAGTGGTTCTCTTGCCTCAGCCTCCTGAATAGCTGGGACTA CAGGTGCACGCCACCACGCCCAGCTAATTTTTGTATTTTAAGTTGAGACGAGGTTTCACCATGTTGGCCT GGTTGTTGTCAATCTCTTGATCTCATGATCCACCCGCCTTGGCCTTCCAAAGTGTTTAGATGACAGGTGT GAGCCACCGCGCCCAGCCTCTATTCTATTCTATTTTGTTCTATTTCTATTACAAGCCAGTAAGCAAGAAA ATATCATAATTTATAAGGAACCCTATAAAAAACAGACAAGCCAAGGGTCTGTCATTAGGAAGTATGCCTG AATAAGAAGCTGAAGATTTTTAGACACAGGTTTCAGGCAACACTGTCTTTAGAGGCTAGGCTCTGGCTCC AGCTCCCTCCAGCCTCCTGTGAATAACAGGCAGGCTTACTTGCAGGTGCCACTTTCCTGGACAGTGGTGG TTAAAGGACAAGGCCCAGAAAGTGCTGAATTAGGTGCCCTTGTTACCGCTAATGTCTTATTGATGACACT ATCTTAGAGCTCTTTTGACATCTTGGCTCTGCGTCTTTTTTTTTTTTTTTTCTTGAGATAGGGTGTTGCT TTGTTATCCAGGCCGGAGTGCAGTGGTGTGATCATGGCTCACTGTAGCCTTGACCTCCTAGACATAACCC ACCTCAGCCTCACAAGTAGCTGGGACCCCAGGCACGCACCATCATGCACAGTTAATTTTTGTGTTTTTTG TAGAGACGAGGTTTCGCCATGTTGCCCAGGCTCATCTCAAACTCCTGGCCCAAACTGTCCTCCCACCTTA GCCTCCCAAAGTGTTTGGTTTATAGGCATGAGCCACTGTGCTTAGCCTGAGTCCCTCTTTTAAACAAACA AAATGGTAAATGGAAAGGAGGAAAGGCTTAAGAAAAAAGATTGAAGCCAGGATTTGTTGTAAGCAAGGAG TAATAAAGGGCAGTTCATTTAGAGAAAGGCATATGACCACCTTTCCCCCTCCAATCAGAATCTAGAAAGT GATTGAGGCCGGGCGCAGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGCGGATCAC GAGATCAGGAGATCGAGACCATCCTGGCTAACATGGTGAAACCCCGTCTCTACTAAAAATCGAAGAAGTT AGCCAGGCGTGGTGGTGGGCGCCTGCAGTCCCAGCTACTCGGGAGGCTGAGAGGCAGGAGAATGGCGTGA ACCTGGGAGGTGGAGCTTGCAGTGAGCCTAGATAGTGCCACTGCACTCCAGCCTGGGCGACAGAGCAAGA CTCTGTCTCAAAAAAAAAAAAAACAAAGCGATTGAGAAAATCAGGTCTGTGTGACCTTAGCAATGAGTTA TTTAGCTTGGGCCACTGTTAGCTTAAGTCAATAACTTCAAGTTTGCGTTGTAGTTGGAATCAATAGAGGA AAAGCTCTCAGCATTACCACATATATCAGAATGTGACATTGATTGCCAGACCAGCCTTATCCAAACACAA GTCCTAGGCTTTTTGCCCTGTTTATGAGCTTTATATGCTGAGGGTATTTGATGAGTCTTAGGGAAAAAAG AACAGCCCTGGGGACACAGCTGCTTTTATGATGAGACATGTTTGCACCCATACCTTAATGGGTTTTGGTG GCAATATTCTGAAATTTGCCACCTACATTTCAAAGATTTGCCCTTTGGGTGAATTAGTGCTGTAGTAGAA GTGGGTGGAGGCTGAGGAGGTTGGATTAAGCAGGTAGAGGATTTCTCAGTGCATGGATCGTGCTGAGGAT GGAGATAGAGCTCTAAGACATCCACGGGCCTTTCCTGAGTGATCAGCTTTGGCTCCTGGGCAGGGGAATT GGAGCTGGATTCTAGTGTGGGAGCACGCTTGTCATCTTCCTTCTTTTCCCCCAGTACCATGCGGAGACCA TCAAGAATGTGCGCACAGCCACGGAAAGCTTTGCTTCTGACCCCATCCTCTACCGGCCCGTTGCTGTGGC TCTAGACACTAAAGGACCTGAGATCCGAACTGGGCTCATCAAGGGCGTGAGTATTCTGCGGAGAGCGAGG GGAAGGCTCAGTAGGCAATATGCCCCAGAGACATGTCCTCCAAAGCGCTGGGTTGCCATGTTTCTTCCCA GTACTATGAAGGACTGCAGAGGAGTTGAGGTCTACAAATGAGGATTTATTCATCACTGTAAACAATGTTG ATTTGATCTACTTTGCTAGGAAATGGTACCACAAAGGAACCTTTTTTTTTTACCCTAAAAACCTAAACTT TAGGCTTTCTAACTTGGAGAACCATCTCTTTGTATCTTTTTCCCCATCATTAAGTAGCATAACTGAAACA TATTCTTTTCTTGGATTATTTCCGTGAAGTATACAGAGTTAGAGAATAAGAGCAAAAAACTGTATTACTT TTAGCAGTGACTTGAGCATTGTTCCCGGGAGGAAAGAGCTTTTCCATTCCTTCTGAGGTGATGCTGCTAC TGGTGTCTCCAGTTTGGACTCTTGCTTACTCTCTTGTCCCTAGAGCGGCACTGCAGAGGTGGAGCTGAAG AAGGGAGCCACTCTCAAAATCACGCTGGATAACGCCTACATGGAAAAGTGTGACGAGAACATCCTGTGGC TGGACTACAAGAACATCTGCAAGGTGGTGGAAGTGGGCAGCAAGATCTACGTGGATGATGGGCTTATTTC TCTCCAGGTGAAGCAGAAAGGTACGTATGGGAGCTGGAGTCCAGTTGTCTAAAACAGTCTTTTGTCTCTA AACTTCCTTGACACAAGGAAGATGGGAAGGTTGGTTGCCTGGCAGTGAGATTGAGTCTGTGTGTTCTCAG GAATCCCTTTTATAACTCATTTATCCTCAAAGATAGGCTTTAATCCAGCATAGTTACATTCTTCTGGTTC TGGAGAACACAGGAACATACATACATATATATATATATATACATATATATATATATATATATATATATAT ATATATATATATATATTTGTTTCGCTGTGTTTTGTTTTGTTTTCAAGACAGAGTCTCGCTCTGTTGCCCA GGCTGGAGTGCAGTGGCATGATCTTGGCTCACTGCAACCTCTGCCTCCAGAGTTCTAGCTATTCTCCTAC CTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCCGCCACCACACCCGGCTAATTTTTTTGTATTTTTAG TAGAGATGGCGTTTTGCCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATCTGCCTGCCTT GGCCTCCCAAAGTCAGAACAGTCTTAATTATCCTTATTTATGGGTGAGGAAAGTGAGGTACAGAGAGGTT AAATGGCTTGCCCAGGATTACACAGTGTAGTAGGTTTTCAACTCTGGTAAAACAGCTCCAGCACCCATAA TGCACCACTTCCCAGCTCACTGTCCTTGCGGGAAAGGTGCCTGCTTCCTGTTGACCTGTGCCCTCGTGCT CTGCCTCCCCTACTTACCCTTTTTCATACAGGTGCCGACTTCCTGGTGACGGAGGTGGAAAATGGTGGCT CCTTGGGCAGCAAGAAGGGTGTGAACCTTCCTGGGGCTGCTGTGGACTTGCCTGCTGTGTCGGAGAAGGA CATCCAGGATCTGAAGTTTGGGGTCGAGCAGGATGTTGATATGGTGTTTGCGTCATTCATCCGCAAGGCA TCTGATGTCCATGAAGTTAGGAAGGTCCTGGGAGAGAAGGGAAAGAACATCAAGATTATCAGCAAAATCG AGAATCATGAGGGGGTTCGGAGGCAAGTCCCCGTTGTCCCTGCTCCAGTCCCAGCGCAGCTCTCCGAAGG GCATGGTCCATCCTGTGAATGTCTGATTCCCAGCCCCTAGCCCATCAGAATGTAGACTCCCAAGCCAGTT CCAAACCTGCTGAATCAGAATATCTTAGGAGAGTAGAAGGCATTATGTTTTTTTGTTTTTGTTTTTTTGT TTTTTTTAAAAAAAAGCTTCCCAGGTAATTGAGATGCTGGCAGCTTGACATTGTTCCCTGGGCCTGGGGA CCAACATTTGAGAGAACAGGGTCACTGCTCACAGGACCAGGGGCCATGATGTTCTGTTCCTGATCAGAAA CACTACCAGTGTTTGCTGGAATGGGGGGACCAGGGGGAAAGATGACAGCAGACACTTAAGAAAGGGCTCT TTTTGGCCCTTCCTGGGGAGCCATGTGGAATTTCAGGGCCTGGTGTCCATGTTAAAGCTTATGGCCTCCT GGTCTTCACTTAGAATGCAGCTGGCTCAGTGATCATGCTAACTCTGGTATGGTCCATTCCACTCTCAGAG GAAGATGTGTGGTTCTTCTCCAGTTTCAGATTGCCCCAACTTAGCTTACCCCCTCCCCAATGCTCACAAA GTAGAGCCCAGTGGGCATGGCCACCATTTTTGGCATCCTGCTAGGAATACAACTCAGCACAACTAAGATG CTAGACACACTCTTGTGGATTAGAAGTGTGTTTGGGGAGGGTGGGGGAGCAACCCTGTGCACCCACTGTA GTGGCCTTACTGTCTGAGCTTTGTGTAGATATCCTCTGTACCAGGCAATTTGGGGTCCTCCCCTTTGCCA TCCTGATAAGCCATAGGCTAGCTGAACTTGGCCCTAGGCCAGGCAAAGCCACATTCCCTCTTGCCTTCAG CAGGTTGGAGTGGGCCACCTCAAAGGGCAGTCCTCAAGTGTCCTTGACTAGATGAGGCCATGGGTCTTTG TGGTGGAAGCAGTCATCAGGCCTCAGGTTCCCTGTCTTGAAGTGCTGATTGGAAAATGGAGGCCCTAGAG AGACCCCTAACATGCATGGGATTTGGAGAGGAGACCTTGGGAATGAGCCCATTTGGATTTGCCCTCTCCC CTTTCTTCCGTCAATGAAGCATCCATATTGGTGTTGAAGCCCAGCAGGCAGAATTGTTGGCCCACTCTGG GGGCCTAAGGTAGCTGGACTGCCTTGCCATCTGTGTGCACCCATGATGATATCATGGATGTCTGTCCTGG TACAAGGACATCTAAGTTAGGGAATCCCAGGGAAACTTCTTGTCTACTGCCATACTTGTGGCCTCTGTTC TATATAACCTCTCTCCCCCCAACTTTGTCCATCAGGTTTGATGAAATCCTGGAGGCCAGTGATGGGATCA TGGTGGCTCGTGGTGATCTAGGCATTGAGATTCCTGCAGAGAAGGTCTTCCTTGCTCAGAAGATGATGAT TGGACGGTGCAACCGAGCTGGGAAGCCTGTCATCTGTGCTACTCAGGCATGTGCCCACCCTTCCCCACAT TCTCATGTGCACACTCGCATGTTTGTATGGGAAAGCTCTGGAGGCTGTCTGATCTCTTCCCATGGAATTG TCGCACGTAACACACAGATAATCCCCTTCCCCCATGTACCTACACAAAGCCATACTCTGTGTACCTACTC ACTATCCAGAGGATCAGCTTGCTGTCATTTGTCTCTGAAGACAGCTCAAGCTACATCTCACTAATGCTCT GTCCCCTCCCAGATGCTGGAGAGCATGATCAAGAAGCCCCGCCCCACTCGGGCTGAAGGCAGTGATGTGG CCAATGCAGTCCTGGATGGAGCCGACTGCATCATGCTGTCTGGAGAAACAGCCAAAGGGGACTATCCTCT GGAGGCTGTGCGCATGCAGCACCTGGTGAGTTCTGGGGCCTGCCCCATCCCCCAGGGCTTCGGACTGGGC CTGGGATGGATGCAAGCTCTGGTGCAGAGCTTTTTAGGTTTCTCCATCCTCTTATGCACAGCCTTTCATT ATCCTCCAAGTTACAGCAGCAAGAGGGTGGGGGTGGAAGTGGAGGTGGCTTTTTTTTTTCTCCTGTTCTG CATTCCTGCCCACACCCCCACCCCTCCATTTCCTTCTGCTCTGGAGGCATCCTCCTTCATTGGACACCAC ACAGTTTATTTCACTTCTGACTTCAAGGTTGTGAATTCTTCCCATGGCTTAAGTCCTGGGATACTTCTGC AGTGAAAGGAGGTCTTGTACCTCTTCCTCAGAGTCAGAAGTTCTGAGTACCTTTGCCCTATTCTGAAAAG GGCTAGGGGCTCCTGCTCCCAGCTGCCCTCTTCCTTTGGCTTCCAATTCAGTTCCCTCTGCCCCGCATCC TGCAGACAGGCGCTCCCGCAGGGGGCCCTTGTGGACCTGCACTGGAGTCTGTTGCCTTCACTGAGCTGCC TGTGCTGGCCTTGCATGGTGCCTGTAGGGGGATTTGCTTTGCTGTGCCATTGGGGTACAGCTGCTGCTCT TACTCTAGACCAAAAAGTCGGGTTGAGTGACTGGTGGCAGGGCCACAGATAGAGACAGCGGGGAGGGTGG CTGACCCTGGCGGCCCTGGACTGAGCGTCTGGAGGAGTCGTGGAGGCTCTTTCCCTTCTTTCTCCTCTGA GAGCTCGTTCTTCAGGCTCTTCCAGCTTGTCATGTCGAGTGCCTGGCCACTGCTCAGGGTTGGAGGCTCA GTCCCTTTGCCCTGTCTGTTCCAGCTCTGGAGCTAACTCAGGGATCCCTGATCAGGGTTACATAGGTTTG GTAAAATGAGTGCTGGAAATTAACTTTCTCCCAGTAGTCTTAGGTCATGCTCAGTGAACTTAAACTTTAT CCAGATATGGTTTTCCTTCAGCCTTTCTATTCCCTTTCTAGCCAGTGAAAGACCCGCTGCCCTTTGACCT CAGCCCCTCCAAGCCCCCAAGTTTAAAACGCCACCCCCTGCCACCAGAAAAAACAGAAAAAAAAAAAAAA AAAAAAAACTAAAACACCCATCTGGTCTGGGCATCTTCCTTTCCTTTTTCACTATGTATCCTGTTACTGG GCTTAAACAGCTTTCAGAGAAGAGATGTCATTTCTATTAAATGCTCTTTCAGTAGCGAACTGAGTTCACA CTTGACTAAGGATATTTTCCGGACTGTCTGTCATCAGCATCCTTAGTGGGTTTCCCCATATTTAAATTGG TAGAGGCCAGGGATGGTGGCTCACACCTGTAATCTCAGTACTTTGGGAGGCCAAGGTAGGTGGATTGCTT GAGCTCAGAAGACCAGCCTGGGCAACCTGGTGAAACCCTGTCTCTACTAAAAATTCAAGTTAGCTAGCTG GGCATGGTGATGCACTTCTGTAGTCCCAGCTACTTGGAGAGGGGGTGATGCTGGGGCAGCAGGATCGCTT GAACCCAGGAGGTTGAGGTTGCAGTGAGCCAAGATGGTACCAGCCTAGGTGACAAAGTGACACCCTGTCT CAAAAAAGAAACCAAACAAACATAAAAAAAAAAACAAAAAAATCGGTAGAGAGTGATTTCTCTCCCAGGC CCACTTAATGTAGACTGGGCCTGGCTGACACCTCACCATTCGTGTGATGTGATTGCTGTTCTGATGCTTA GATACTCTTGGCGCAGTCTCACAATTGCCACCATGGTAGGAAGGTGTCCCAGGAGACGGTGCACCTTGAA CCAGTCACCACTAAAGTGGCTGCCTTTCTGGGTCTCTCCACACATCCCCTCTCTCTAATTTCCCTACTTA ATCGTGTGACTTCATGGTCTCAAAGGAGGAACAGAGGCTGATCTTGACTTAGATATACTGAACCATGAAA TCACTGCATAGAATGTGGGGACTTGAATGTGTCTTTGGGCAAGTCATTTAACCTCTTAAGACCTCATCTG TAAAATGGATTAGATATGTTTAATTATAGCCTTAGCATTAAATATTCATTGCTGTTATTATTAAGTGTCT GATAAGTCTCTGTGTACATGGATGTAATCTTCCTAACTCCCATTACCTCCATTTATAGATGAGGGTTATA TGGCCAATAAAGCCTGGGTTTGAATCTAGGTCTACTGCCTCCAAAGCCAGTCTTCTCTCCTGCAACATCA TGCTCTGTCTAGCAGGAGATGAGAACAGGTCTCCATTTGGAGCCTGTCAGTGGGGTCAGAGACTAAGATT CAGGCTCAGGGTCTAAATTCCATATCCTTTCTTCCATACCCTGGTGTTTCCTATGAACAGATAGATACTT TAGGGCTGCAAGGTTTGGATTGCATGGCACTGCTCAGAAGATAAGTTACAGGTCTGGGCTAGGCTGTAGC TGCCCCTCCAGGTGGCTAGACCTTTCCTTTCTGTGTCACCAGTTAACACTGGCCAACAGTTCCTTCCATT AACTGTTCACTGCTTTCTCCTGTGTCTAACTGATGCAGTTTATGACCCATAACTAAGAGCAGTACCAGGT ATGGCTCTGTTTCCTGTTCATGTCCCCTGTCCTCTGGGCTGCATGCATTCCGTTCTTACAGAAAGAATAC CTTTAACCTAGTACATCCTGCCACACATCTGCTTCTACTGTGAAATTGATGAGGGGGTATTACCGATTCT TCCCTCACCCATCATTTACTGAGATGCTGGTGATTGCATTATAATCCTCTAAAGCTTACATTGTCTTTCT GATTCTTGGTCTTATCTGAGCAAGTGATCTATAAATAACTCAGTGGCTTTCTCATGACTGTTTTAATTAT TAGATTTTAATCAAGTGTCTTATTAAATATATCTGCATGCTTCCACAGGCATCTGTCTCTTCACATGGCT GTTCAGTGTGCCTCTCACAAGTTAGCCCACGTTTTCTGTTCTCCTGCTTCAAACTCAGTTGAGCTGCCTT GCTTTGGCTTTGATCCCAGCTTTCCAGCGCTGCTCAATCTGTTGCCATGGCAGGCCATTGGAAAGGCTCA GTGCATCCCCGTGCCTGAAGCCAAGTGAGCGCTCACTCCATGCATGCATGGAGGCTGGGCAGGAGCCTGC CTAATCAACCAGCCATGTGAGGAGGGAGGGCCTGTTCCTTCCTGTAAGCTATGTCATGAGGCAGCGTGGT CAAGTCCTCTGCCAGGGAGTGGCCTGGGCCCAGCCTGGGCATGTTTTCATGCCAGGGTGCTAGAGCCTAC TGCCAGATTGTCTCCCTCCACCCCCAATGAAAAAATCCTTCCAGAAGGGAAGAGCCAATTTCCCCTGTAT TGGAGGGGAAGTGGCAGCACCTCCTGAAGCAGTTGGACTTTCATCACCCTACCTCTGCATCTGCCTGAAG GACAGATTTAGCCAATTAACCTAAGGTTACCTTCCTCTCTGATAAATTCCCCATTCTGTCTTCCCATGTG TTGTGTCTCGTTTTTTTCCTCCTCCTTCCCTCTTCCTTGCCCCCTCTTCCCCTAAACCTTACAGATAGCT CGTGAGGCTGAGGCAGCCATGTTCCACCGCAAGCTGTTTGAAGAACTTGTGCGAGCCTCAAGTCACTCCA CAGACCTCATGGAAGCCATGGCCATGGGCAGCGTGGAGGCTTCTTATAAGTGTTTAGCAGCAGCTTTGAT AGTTCTGACGGAGTCTGGCAGGTAGGGCCCTAAGGGCAGGTAACACTGTTAGGATAACCAGCCTCTTGCT CCACCTGCTCTAGGAGAAGACAGCCAGGCCCAACCTGGCATCTGGGCACAGAGCCTCTTCTCGTCTGTAG GAACACCGCCAGGGAGGTCATGGCAGGGCAGGACCAAAGGGTCCTGTGGCTCAGTAGGCACAGTAGATGT CACAGGCACTTGGTGAAGGACTGGTTTCTGTGGAGTCTTGATCTTGGCTCAGCTCAGAATCTCCAGTGAT TGGGCTCCTCTTGGCCTTTGTTCCCAGGAACATGTTCCTCACCAGCTGTCCGGTGACTCTTCCCCTCCCT CTCCTTTTGTGACAAAGCTCTGACAAAGCTCTGTCCCCCTCTCGTCCCTCTGGACGGATGTTGCTCCCCT AGATTGCCCGTGAGGCAGAGGCTGCCATCTACCACTTGCAATTATTTGAGGAACTCCGCCGCCTGGCGCC CATTACCAGCGACCCCACAGAAGCCACCGCCGTGGGTGCCGTGGAGGCCTCCTTCAAGTGCTGCAGTGGG GCCATAATCGTCCTCACCAAGTCTGGCAGGTAGGAGGCGGCAGCGGCTCCCTGGAATGCCCTGCTCAGTG GTACCTCACCTTGGGGGTCCTGGGAGCAGTCCATTGAACAATGCTCAGGTGGCACTGAGCCAAGGTAAGA CCCCTCTGCCTGCCACCTTGGGCCTGCAGGGAAGGATTGAGCAGAGCCCCTTCCCTGGGCCCAAAGGACT CTAGGTAGCACTCATAAGGAATGTCAGAACATTTGGATCAAAAGCAAATTTATGCTGGAGATTTATTACA TAACAGTGCACAGGCTGACTACAAATGGTTATTTGATATTGAAAATTTAGTCCTCTAAAATTGTAAAAGA TAACCACTTTTGCTTATTCCAGTTACTATGTGCTCTTTAAAAATTTCAGTTGGGAAATGAATTTATTTAA ATGCTGTTTACTGTGCCTCCATTTGGCACACTAGTCCCTGCTGTTTTTGAGCCCTAAAGACAAATTGGGT TCCAGCTCAGGAGAGGTTGCTGTGCTATCTTGGCTGACATTCTGTGGGGCCTGGCAGCCAGGCTGAGGAC TGTGTGGCCTATGCTGGGCCTCCAACTTGGGATCCCTTCCTTGGCCCAGGACATTGAGTTAATGTCCTTC ACTCTCCTAGTTAGGGAGTATGCTCCTTGTCCCTGTCCACAGGGCAGCAAGGGTTTCCTGGAAGAGGGGA GCAAACAGGCAGTGCCCATGCACTGAGGAGCAGCAGATGGGCGTGGGCAGCCCAGAGAACCAGGACACAA GCTCTGTGCAGATGCCCTCAGCAGAGGGCTCCAGCCTCCCACTCTTGGCTGAACAGCTCCAACCCGTAGG GTTGACCTTTCTTAAAAGGTCCAGTTCTTGCTGTTTGGCTATTTTAAGCTCTAGTCTTCTGGGGTTTCAC TCAGCTGGTCCTGGCTTCAGCAATTGCTTCCCTCTGAAGGCCTTGCATAGAGGCCAAGCGTGAAGTGCAG GGACTTCTCTGCTGTGATGTGGCTTAAGTTTCCCTGACACCTGTTGAGTGTCCTCATAACTTCCCTTCTG GTGCCCCTCCCCAGCTCCTGAGACACAGCTGCAGCTACAAGTGTGCAGTGTCAGTGTTCAAGAAAGTGCC TGGCAGAGGGGCTTTAGAAGGGTCCCCTGCCTTCCAAAGGAGCTTTGGCAGGCAGAGCTGCTCCTGCAGC AACACTCCCATTTCCTGTTCTTGCCTGCTGAGTAGCACCTAGATTTCTAAGCCTCATCTAGATACTCAGA TTTGATTCTGGGCCTTTATAGCCCAGTTGCTGGGACTGTTTCAGGAGCTAGGGGCCATGTGGGGCAGGGA GAGGGCACAAAAGTAGAGAAGCCTGATGTTGATTCCCAGGGGGCTGGTCAGCTCTGCTACTGCTCCTTGC AGATGTCAAGAGTCAGGTGCTAGTCACGTGCTGCTTGGCTTGTCACTGTCATTGGCAGCGAGAGGAATGG GTGCTGGTGACATTGGGCCAGGGCTGCCTCTCTGTGTCAGAGTTCAGGGTGTAGGAGGGGTTCTGCCAAC CATGGGCTGTGTGGGGTAAGTGGGTGAGGCTGATCTTGCTGGGTCAAGGTGATCCTGAGCCCTTGGCCTG TGGAATGGGGGTAGAGGGCAAATGGTAACCTAGCATGCTGTGGGGGATATAGGATGAGGGGCTGCCCGAG CCTCGGGAGGGGTCCTAGGGAGCAGATGTTGAAGAGGCCAGAGCCCTCAGTGAGCTGGATGAGAGGGTGA GCTGTTTGAACGCCCTGAGGGTACTTCCTGGGGCCTCGTGTAATGGTCTCTTCTGTATGTCCCCCATCCC ATCTCAGGTCTGCTCACCAGGTGGCCAGATACCGCCCACGTGCCCCCATCATTGCTGTGACCCGGAATCC CCAGACAGCTCGTCAGGCCCACCTGTACCGTGGCATCTTCCCTGTGCTGTGCAAGGACCCAGTCCAGGAG GCCTGGGCTGAGGACGTGGACCTCCGGGTGAACTTTGCCATGAATGTTGGTACGTGGCTGGAGCAGGGGC TAGAGCCTAGAGGAGCTTGGGGATGCTTGAGCATTGGCTTCTGTGGGACCCCGAAAGTTTGGGGAATAGA AAGGGGAACACACAGACCTTAGTGGGGCAAAAGGCCCAGCGACTGTTCCTCTCCCTTATTGGGAATGTTC ATTCTGAATCTCTCATTCTCCGAAGTCCTAAGCTGAGCCAGGAGGGAAAAGGGTCCTTTGAGTTGTAGGG CTGAGCAATTCAGTTCCTCTTCTCTTCTAGTCTGGGGCTCAAAGCAAAATTGTCCATTTTTTGGCATCTG CTCATTACTGAGAGTTTTTTTTGTTTTTTGTTTTTTTTTTAAATAAAATTGGCCACAGCTCCTGTGCTGT GGGGTGGCATACACAGATTACGTACTGATGTGGCCATTGTCCCTGTATAAGGTAGGGTATCATCAGATGA CAGGAAGCAGCTAGCTCTGACCCTGGGCAAGGCTTTGCACCCTCTCCAGGATAGTGAATGATGTCCAAAG GTCCCTGCCAACCCTGCCATCTGAGTGATAAGGACATTTCAGGGCCTTCCTCCTGTTTGCCTGGGCTGTG AGTTTGGTGCCACCTTGTGGTGTGAGGAAGTAGTGGTCAGCCAGCCTAGTTCAGTACTCAGGCTATGGGG CAGCTGCCCAGGTGCAAACCTGCCTGGCTTGGCTTTTACTCACCAACCTCCCTTCTCTTCCTCCAGGCAA GGCCCGAGGCTTCTTCAAGAAGGGAGATGTGGTCATTGTGCTGACCGGATGGCGCCCTGGCTCCGGCTTC ACCAACACCATGCGTGTTGTTCCTGTGCCGTGATGGACCCCAGAGCCCCTCCTCCAGCCCCTGTCCCACC CCCTTCCCCCAGCCCATCCATTAGGCCAGCAACGCTTGTAGAACTCACTCTGGGCTGTAACGTGGCACTG GTAGGTTGGGACACCAGGGAAGAAGATCAACGCCTCACTGAAACATGGCTGTGTTTGCAGCCTGCTCTAG TGGGACAGCCCAGAGCCTGGCTGCCCATCATGTGGCCCCACCCAATCAAGGGAAGAAGGAGGAATGCTGG ACTGGAGGCCCCTGGAGCCAGATGGCAAGAGGGTGACAGCTTCCTTTCCTGTGTGTACTCTGTCCAGTTC CTTTAGAAAAAATGGATGCCCAGAGGACTCCCAACCCTGGCTTGGGGTCAAGAAACAGCCAGCAAGAGTT AGGGGCCTTAGGGCACTGGGCTGTTGTTCCATTGAAGCCGACTCTGGCCCTGGCCCTTACTTGCTTCTCT AGCTCTCTAGGCCTCTCCAGTTTGCACCTGTCCCCACCCTCCACTCAGCTGTCCTGCAGCAAACACTCCA CCCTCCACCTTCCATTTTCCCCCACTACTGCAGCACCTCCAGGCCTGTTGCTATAGAGCCTACCTGTATG TCAATAAACAACAGCTGAAGCACC Download Select all
Length	32784
OMIM ID	179050
HGNC ID	HGNC:9021
Unigene ID	Hs.534770
Ensembl ID	ENSG00000067225
PharmGKB ID	-

mRNA Information

mRNA ID	XM_005254445.3
Exon	join(1..489,12703..12869,14313..14404,21335..21466, 21954..22140,22922..23192,24536..24686,24933..25085, 29193..29359,31158..31339,32057..32784)
mRNA Sequence	>5315 AGTCCCACCGAAAGGGCAACCTGCCCGCGCGTTCCGCCACCGCCGCCGCGCTTCCTCCTGAAGG TGACTGCGCCCGCGGGGACGCAGGGGGCGGGGCCCGGGTCGCCCGGAGCCGGGATTGGGCAGAG GGCGGGGCGGCGGAGGGATTGCGGCGGCCCGCAGCGGGATAACCTTGAGGCTGAGGCAGTGGCT CCTTGCACAGCAGCTGCACGCGCCGTGGCTCCGGATCTCTTCGTCTTTGCAGCGTAGCCCGAGT CGGTCAGCGCCGGAGGACCTCAGCAGCCATGTCGAAGCCCCATAGTGAAGCCGGGACTGCCTTC ATTCAGACCCAGCAGCTGCACGCAGCCATGGCTGACACATTCCTGGAGCACATGTGCCGCCTGG ACATTGATTCACCACCCATCACAGCCCGGAACACTGGCATCATCTGTACCATTGGCCCAGCTTC CCGATCAGTGGAGCTGAAGAAGGGAGCCACTCTCAAAATCACGCTGGATAACGCCTACATGGAA AAGTGTGACGAGAACATCCTGTGGCTGGACTACAAGAACATCTGCAAGGTGGTGGAAGTGGGCA GCAAGATCTACGTGGATGATGGGCTTATTTCTCTCCAGGTGAAGCAGAAAGGTGCCGACTTCCT GGTGACGGAGGTGGAAAATGGTGGCTCCTTGGGCAGCAAGAAGGGTGTGAACCTTCCTGGGGCT GCTGTGGACTTGCCTGCTGTGTCGGAGAAGGACATCCAGGATCTGAAGTTTGGGGTCGAGCAGG ATGTTGATATGGTGTTTGCGTCATTCATCCGCAAGGCATCTGATGTCCATGAAGTTAGGAAGGT CCTGGGAGAGAAGGGAAAGAACATCAAGATTATCAGCAAAATCGAGAATCATGAGGGGGTTCGG AGGTTTGATGAAATCCTGGAGGCCAGTGATGGGATCATGGTGGCTCGTGGTGATCTAGGCATTG AGATTCCTGCAGAGAAGGTCTTCCTTGCTCAGAAGATGATGATTGGACGGTGCAACCGAGCTGG GAAGCCTGTCATCTGTGCTACTCAGATGCTGGAGAGCATGATCAAGAAGCCCCGCCCCACTCGG GCTGAAGGCAGTGATGTGGCCAATGCAGTCCTGGATGGAGCCGACTGCATCATGCTGTCTGGAG AAACAGCCAAAGGGGACTATCCTCTGGAGGCTGTGCGCATGCAGCACCTGATTGCCCGTGAGGC AGAGGCTGCCATCTACCACTTGCAATTATTTGAGGAACTCCGCCGCCTGGCGCCCATTACCAGC GACCCCACAGAAGCCACCGCCGTGGGTGCCGTGGAGGCCTCCTTCAAGTGCTGCAGTGGGGCCA TAATCGTCCTCACCAAGTCTGGCAGGTCTGCTCACCAGGTGGCCAGATACCGCCCACGTGCCCC CATCATTGCTGTGACCCGGAATCCCCAGACAGCTCGTCAGGCCCACCTGTACCGTGGCATCTTC CCTGTGCTGTGCAAGGACCCAGTCCAGGAGGCCTGGGCTGAGGACGTGGACCTCCGGGTGAACT TTGCCATGAATGTTGGCAAGGCCCGAGGCTTCTTCAAGAAGGGAGATGTGGTCATTGTGCTGAC CGGATGGCGCCCTGGCTCCGGCTTCACCAACACCATGCGTGTTGTTCCTGTGCCGTGATGGACC CCAGAGCCCCTCCTCCAGCCCCTGTCCCACCCCCTTCCCCCAGCCCATCCATTAGGCCAGCAAC GCTTGTAGAACTCACTCTGGGCTGTAACGTGGCACTGGTAGGTTGGGACACCAGGGAAGAAGAT CAACGCCTCACTGAAACATGGCTGTGTTTGCAGCCTGCTCTAGTGGGACAGCCCAGAGCCTGGC TGCCCATCATGTGGCCCCACCCAATCAAGGGAAGAAGGAGGAATGCTGGACTGGAGGCCCCTGG AGCCAGATGGCAAGAGGGTGACAGCTTCCTTTCCTGTGTGTACTCTGTCCAGTTCCTTTAGAAA AAATGGATGCCCAGAGGACTCCCAACCCTGGCTTGGGGTCAAGAAACAGCCAGCAAGAGTTAGG GGCCTTAGGGCACTGGGCTGTTGTTCCATTGAAGCCGACTCTGGCCCTGGCCCTTACTTGCTTC TCTAGCTCTCTAGGCCTCTCCAGTTTGCACCTGTCCCCACCCTCCACTCAGCTGTCCTGCAGCA AACACTCCACCCTCCACCTTCCATTTTCCCCCACTACTGCAGCACCTCCAGGCCTGTTGCTATA GAGCCTACCTGTATGTCAATAAACAACAGCTGAAGCACCAAAAAAAAAAAAAAA Download Select all
Length	2294

CDS Information

Location	join(826..917,12703..12869,14313..14404,21335..21466, 21954..22140,22922..23192,24536..24686,24933..25085, 29193..29359,31158..31339,32057..32163)
CDS Sequence	>gi\|332164776:285-1658 Homo sapiens pyruvate kinase, muscle (PKM), transcript variant 5, mRNA ATGTCGAAGCCCCATAGTGAAGCCGGGACTGCCTTCATTCAGACCCAGCAGCTGCACGCAGCCATGGCTG ACACATTCCTGGAGCACATGTGCCGCCTGGACATTGATTCACCACCCATCACAGCCCGGAACACTGGCAT CATCTGTACCATTGGCCCAGCTTCCCGATCAGTGGAGCTGAAGAAGGGAGCCACTCTCAAAATCACGCTG GATAACGCCTACATGGAAAAGTGTGACGAGAACATCCTGTGGCTGGACTACAAGAACATCTGCAAGGTGG TGGAAGTGGGCAGCAAGATCTACGTGGATGATGGGCTTATTTCTCTCCAGGTGAAGCAGAAAGGTGCCGA CTTCCTGGTGACGGAGGTGGAAAATGGTGGCTCCTTGGGCAGCAAGAAGGGTGTGAACCTTCCTGGGGCT GCTGTGGACTTGCCTGCTGTGTCGGAGAAGGACATCCAGGATCTGAAGTTTGGGGTCGAGCAGGATGTTG ATATGGTGTTTGCGTCATTCATCCGCAAGGCATCTGATGTCCATGAAGTTAGGAAGGTCCTGGGAGAGAA GGGAAAGAACATCAAGATTATCAGCAAAATCGAGAATCATGAGGGGGTTCGGAGGTTTGATGAAATCCTG GAGGCCAGTGATGGGATCATGGTGGCTCGTGGTGATCTAGGCATTGAGATTCCTGCAGAGAAGGTCTTCC TTGCTCAGAAGATGATGATTGGACGGTGCAACCGAGCTGGGAAGCCTGTCATCTGTGCTACTCAGATGCT GGAGAGCATGATCAAGAAGCCCCGCCCCACTCGGGCTGAAGGCAGTGATGTGGCCAATGCAGTCCTGGAT GGAGCCGACTGCATCATGCTGTCTGGAGAAACAGCCAAAGGGGACTATCCTCTGGAGGCTGTGCGCATGC AGCACCTGATTGCCCGTGAGGCAGAGGCTGCCATCTACCACTTGCAATTATTTGAGGAACTCCGCCGCCT GGCGCCCATTACCAGCGACCCCACAGAAGCCACCGCCGTGGGTGCCGTGGAGGCCTCCTTCAAGTGCTGC AGTGGGGCCATAATCGTCCTCACCAAGTCTGGCAGGTCTGCTCACCAGGTGGCCAGATACCGCCCACGTG CCCCCATCATTGCTGTGACCCGGAATCCCCAGACAGCTCGTCAGGCCCACCTGTACCGTGGCATCTTCCC TGTGCTGTGCAAGGACCCAGTCCAGGAGGCCTGGGCTGAGGACGTGGACCTCCGGGTGAACTTTGCCATG AATGTTGGCAAGGCCCGAGGCTTCTTCAAGAAGGGAGATGTGGTCATTGTGCTGACCGGATGGCGCCCTG GCTCCGGCTTCACCAACACCATGCGTGTTGTTCCTGTGCCGTGA Download Select all
CCDS ID	-

Protein Information

Protein ID	NP_872270.1
Protein Name	pyruvate kinase, muscle
Molecular Wt. (Da)	57931
Protein Sequence	>5315 MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEM IKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSG TAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADF LVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRK VLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRA GKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIARE AEAAMFHRKLFEELVRASSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSAHQVARYRPRA PIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVL TGWRPGSGFTNTMRVVPVP Download Select all
Length	531
Uniprot ID	P12111
Ensembl Protein ID	ENSP00000295550

Post-translational Modification Information (Source : HPRD)

Residue	Type	site
S	Phosphorylation	37;-
S	Phosphorylation	37;-
S	Phosphorylation	37;-
T	Phosphorylation	328;-
T	Phosphorylation	328;-
T	Phosphorylation	328;-
S	Acetylation	2
S	Acetylation	2
S	Acetylation	2
T	Phosphorylation	45
T	Phosphorylation	45
T	Phosphorylation	45
K	Acetylation	266
K	Acetylation	266
K	Acetylation	266
K	Acetylation	62
K	Acetylation	62
K	Acetylation	62
K	Acetylation	89
K	Acetylation	89
K	Acetylation	89
K	Acetylation	433
K	Acetylation	305
K	Acetylation	305
K	Acetylation	305
K	Acetylation	166
K	Acetylation	166
K	Acetylation	166
K	Acetylation	135
K	Acetylation	135
K	Acetylation	135
Y	Phosphorylation	105
Y	Phosphorylation	105
Y	Phosphorylation	105
C	S-Nitrosylation	49
C	S-Nitrosylation	49
C	S-Nitrosylation	49
Y	Phosphorylation	390
S	Phosphorylation	57
S	Phosphorylation	57
S	Phosphorylation	57
T	Phosphorylation	129
T	Phosphorylation	129
T	Phosphorylation	129
S	Phosphorylation	172
S	Phosphorylation	172
S	Phosphorylation	172
S	Phosphorylation	287
S	Phosphorylation	287
S	Phosphorylation	287
T	Phosphorylation	405
S	Phosphorylation	406

Enzyme Information

No matching records could be found.

PPi Information

(Click to view network : Source STRING) View

Interactant Gene ID (Source: HPRD)	Gene Symbol	HPRD ID	Ref Seq ID	Technique	References
5894	RAF1	1265	NP_002871.1	yeast 2-hybrid	12620389
6462	SHBG	1646	NP_001031.2	yeast 2-hybrid	15862967
93145	OLFM2	10127	NP_477512.1	yeast 2-hybrid	16169070

Pathway Information

Pathway Name	Pathway ID
Biosynthesis of amino acids	hsa01230
Carbon metabolism	hsa01200
Central carbon metabolism in cancer	hsa05230
Glucagon signaling pathway	hsa04922
Glycolysis / Gluconeogenesis	hsa00010
Metabolic pathways	hsa01100
Purine metabolism	hsa00230
Pyruvate metabolism	hsa00620
Type II diabetes mellitus	hsa04930
Viral carcinogenesis	hsa05203

Structure Information

No matching records could be found.

Domain Information

ID	Location	Description
cd00288	42-530	Pyruvate_Kinase
cl21481	42-530	Pyruvate_Kinase superfamily
pfam00224	42-395	PK
PRK06739	48-386	PRK06739
PRK14725	38-402	PRK14725
pfam02887	409-528	PK_C
cl19522	409-528	PK_C superfamily
TIGR01064	43-527	pyruv_kin
COG0469	43-530	PykF
PRK05826	43-514	PRK05826
PLN02461	45-512	PLN02461
PRK09206	43-527	PRK09206
PRK06354	30-527	PRK06354
PTZ00300	69-530	PTZ00300
PTZ00066	8-531	PTZ00066
PRK06247	42-527	PRK06247
PLN02765	45-511	PLN02765
PLN02623	37-517	PLN02623
PLN02762	42-531	PLN02762
PRK08187	38-378	PRK08187

Ontology Information

HPRD ID	3632
Molecular Class	Unknown
Molecular Function	Transcription factor activity (GO:0003700)
Biological Process	Regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolism (GO:0019219)

SNP Information

View SNP Geneview Report

Drug Information

Uniprot ID DrugBank ID Name Type
P14618 DB00119 Pyruvic acid SmallMoleculeDrug

External Links

ArrayExpress GO
ICGC GO
Cancer3D GO
COSMIC GO
NCG GO
TSGene GO
Tumor Gene Family of Databases GO
CCDB GO
G2SBC GO
Osteosarcoma Database GO
RCDB GO

ArrayExpress	GO
ICGC	GO
Cancer3D	GO
COSMIC	GO
NCG	GO
TSGene	GO
Tumor Gene Family of Databases	GO
CCDB	GO
G2SBC	GO
Osteosarcoma Database	GO
RCDB	GO